Band deconvolution and Fluorescence quenching Methods for determination of Caffeine content in Coffee Seed
| dc.contributor.advisor | Asfaw Araya (PhD) | |
| dc.contributor.author | Mengesha Menberu | |
| dc.date.accessioned | 2018-06-29T06:40:14Z | |
| dc.date.accessioned | 2023-11-09T11:23:57Z | |
| dc.date.available | 2018-06-29T06:40:14Z | |
| dc.date.available | 2023-11-09T11:23:57Z | |
| dc.date.issued | 2006-06 | |
| dc.description.abstract | In this project,the concentration of caffeine in coffee in water solution is investigated. The molar decadic absorption coefficient in water is determined by preparing several concentrations and applying least square method. The obtained result is 1098.87 m2 mol . The constant that relate the absorbance of caffeine in coffee in water solution to the extracted caffeine is developed and its value is ( 2.9 mg−1). Moreover band deconvolution method is developed to remove the interfering matrix elements in the absorption spectrum of coffee in water solution. To apply this method the interfering band is modelled by a convolution of five gaussian functions. The result obtained using this technique is consistent with other results with an error of 5−8%. Finally the concentration of caffeine in coffee seed is determined by correlating the fluorescence intensity of the emission spectrum of pure caffeine and coffee powder in water solution by applying quenching fluorescence. After all these methods are applicable in every part of the country to determine the concentration of caffeine in coffee seed with least cost using a universal solvent,water. Key words: Caffeine, deconvolution, interfering band, gaussian model, absorbance, UV/Vis, and fluorescence | en_US |
| dc.identifier.uri | http://10.90.10.223:4000/handle/123456789/4833 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Caffeine | en_US |
| dc.subject | Deconvolution | en_US |
| dc.subject | Interfering Band | en_US |
| dc.subject | Gaussian Model | en_US |
| dc.subject | Absorbance | en_US |
| dc.subject | UV/Vis | en_US |
| dc.subject | and Fluorescence | en_US |
| dc.title | Band deconvolution and Fluorescence quenching Methods for determination of Caffeine content in Coffee Seed | en_US |
| dc.type | Thesis | en_US |