Band deconvolution and Fluorescence quenching Methods for determination of Caffeine content in Coffee Seed
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Date
2006-06
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Addis Ababa University
Abstract
In this project,the concentration of caffeine in coffee in water solution is investigated.
The molar decadic absorption coefficient in water is determined by preparing several
concentrations and applying least square method. The obtained result is 1098.87
m2
mol . The constant that relate the absorbance of caffeine in coffee in water solution
to the extracted caffeine is developed and its value is ( 2.9 mg−1). Moreover band
deconvolution method is developed to remove the interfering matrix elements in the
absorption spectrum of coffee in water solution. To apply this method the interfering
band is modelled by a convolution of five gaussian functions. The result obtained
using this technique is consistent with other results with an error of 5−8%. Finally the
concentration of caffeine in coffee seed is determined by correlating the fluorescence
intensity of the emission spectrum of pure caffeine and coffee powder in water solution
by applying quenching fluorescence. After all these methods are applicable in every
part of the country to determine the concentration of caffeine in coffee seed with least
cost using a universal solvent,water.
Key words: Caffeine, deconvolution, interfering band, gaussian model, absorbance,
UV/Vis, and fluorescence
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Keywords
Caffeine, Deconvolution, Interfering Band, Gaussian Model, Absorbance, UV/Vis, and Fluorescence