Prophylactic Effects of Methanolic Extract of Artemisia Absinthium in Trypanosoma Congolense Infected Mice
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Date
2012-06-01
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Addis Ababa Univensity
Abstract
An experiment was conducted to determine the in vivo anti-typanosomal effect of methanol
extracts of the aerial parts of Artemisia absinthium against Trypanosoina congolense. Thirty-six
Swiss white male mice aged between 6 - 8 weeks were divided into six expcrimental groups each
consisting of six animals. Methanol extract of the plant was prepared. T. congolense was isolated
from cattle at Didessa valley (western Ethiopia) and propagated on mice. All groups received 1 x
105 /ml of trypanosomes in 0.2 ml of blood /PBS via intra peritoneal route of inoculation. The
methanol extract of A. absinthium was given orally to three groups. at doses of 400 mg/kg, 800
mg/kg and 1200 mg/kg body weight for 7 consecutive days before in infection. One group
remained as infected non treated control and the other group received Isometamidium chl oride as
treated control. The last remaining group rep resented the non infected and non treated but
received distilled water only. The effect of' the extract on Ievels of parasitaemia, PCV. body
weight, and mice survival was monitored for 70 days. No parasitc was dctected by wet film
examination upto 21 days post infection with T congulense in mice treated with methanol
extracts of A. absinlhium and Isometamidium chloride. Similarly, groups received methanol
ex tracts of A. absinrhium and Isometamidium chloride had significantly higher body weights
than the untreated infected group (P<0.01). Significant higher PCV and mice survival time were
observed in groups treated with methanol extracts of the plant and lsomethamidium chloride
compared to the untreated infected control (P<0.01). It can be concluded that the plant ex tract
has a good prophylactic potential against pathogenic trypanosomes ( T. congolense) of livestock
importance by suppressing further multiplication of the parasite, maintaining good PCV, body
weight, and survival of infected animals
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Keywords
Artemisia absinthium, Isometamidium chloride mice, parasitaemia, PCV, survival time, Trypanosoma congolense, body weight