Molecular Characterization of Introduced Sugarcane (Saccharum officinarum L.) Genotypes in Ethiopia Using Inter Simple Sequence Repeat (ISSR) Markers
| dc.contributor.advisor | Haileselassie, Teklehaimanot (PhD) | |
| dc.contributor.advisor | Tesfaye, Kassahun (PhD) | |
| dc.contributor.author | Tazeb, Adebabay | |
| dc.date.accessioned | 2018-06-14T13:21:27Z | |
| dc.date.accessioned | 2023-11-18T09:50:12Z | |
| dc.date.available | 2018-06-14T13:21:27Z | |
| dc.date.available | 2023-11-18T09:50:12Z | |
| dc.date.issued | 2015-03 | |
| dc.description.abstract | The major thrust of sugarcane (Saccharum officinarum L.) variety improvement programs is to increase sugar yield, while due to its genetic complexity, sugarcane has received very little research interest, despite its economic importance, and molecular techniques are being developed only in recent times. Furthermore, until the present date no molecular studies were carried out to evaluate sugarcane germplasm from Ethiopian fields. Therefore, the objective of this study was to evaluate genetic diversity and establish relationships among populations of sugarcane in Ethiopia using ISSR markers. Genomic DNA was extracted from leave samples of 82 sugarcane genotypes according to modified CTAB method. A total of 149 scorable and reproducible bands were generated using 12 ISSR primers among which 124 were polymorphic and attributed to PPL = 83.22 %, h = 0.31, and I = 0.45 at species level. Intra-population diversity based on polymorphic loci ranged from 28.86 % to 47.65 % with mean of 38.35 %, Nei’s gene diversity of 0.097 to 0.171 with mean of 0.137, Shannon’s information index of 0.147 to 0.255 with mean of 0.205, and within population AMOVA of 63.56 %. All diversity parameters confirm that, the highest diversity was obtained from those that were obtained from France and Cuba whilst the lowest was from those of Barbados and South Africa. AMOVA revealed 36.4 % among population variation which is significantly lower than that of within population variation. From Jaccard’s pairwise similarity coefficient, those from Sudan and South Africa were most related populations exhibiting 0.70 similarity and USA and Barbados were the most distantly related populations with similarity of 0.53. With all clustering analysis, most of the genotypes clustered to their respective origins. Thus, ISSR markers detected a range of diversity from sugarcane varieties with their unique identity that deserve conservation attention and improvement programs. Key words: Commercial crop, genetic diversity, ISSR markers, Saccharum officinarum | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/993 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Commercial crop; genetic diversity; ISSR markers; Saccharum officinarum | en_US |
| dc.title | Molecular Characterization of Introduced Sugarcane (Saccharum officinarum L.) Genotypes in Ethiopia Using Inter Simple Sequence Repeat (ISSR) Markers | en_US |
| dc.type | Thesis | en_US |