Antihypertensive Activity of Aerial Parts of Satureja Punctata (Benth.) Briq. (Lamiaceae)
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Date
2015-02
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Addis Ababa University
Abstract
Satureja punctata Benth. Briq. (Lamiaceae) locally known as ‘‘Lomishet’’ is among the plants
used in Ethiopian traditional medicine for the treatment of various diseases including
hypertension. The current study aimed at evaluating the antihypertensive activity of the
decoction of the aerial parts of this medicinal plant in guinea model of hypertension and its
vasorelaxant effect on isolated aorta. Acute hypertension was induced by surgical procedures
involving clamping of the left renal artery and blood pressure was recorded invasively by direct
cannulation method from the right common carotid artery. Intravenous administration of the
decoction at doses of 10, 20 and 30 mg/kg caused 13.66 ± 0.27, 34.35 ± 0.33, 45.78 ± 0.23%
(n=6) fall in (p < 0.01) MABP, respectively, in normotensive guinea pig and a respective fall of
16.02 ± 0.28, 38.52 ± 0.50, 52.07 ± 0.42 % (n=6) (p < 0.01) MABP in renovascular hypertensive
guinea pig. The decoction also caused a dose-dependent relaxation of aorta precontracted with
KCl at a concentration of 2.5- 40 mg/ml, with a maximum relaxation of 98.19 % achieved at 40
mg/ml. Preparative reversed-phase HPLC analyses of the aerial part decoction of S. punctata
resulted in isolation of two phenolic compounds, rosmarinic acid and linarin. The structures of
these compounds were elucidated by utilization of spectroscopic techniques, i.e., MS, UV, IR,
1D, and 2D NMR. Further, rosmarinic acid showed significant (p<0.01) reduction of MABP by
8.14 ± 0.27, 15.79 ± 0.33 and 31.78 ± 0.64 % (n = 6) at doses of 0.75, 1.5 and 3 mg/kg,
respectively, in normotensive guinea pig. The findings of this study suggest that the aerial parts
of S. punctata have genuine antihypertensive activity.
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Keywords
Antihypertensive;S. puctata (Benth.) Briq