Determination of Fat and Protein Content of Milk using He-Ne Laser Light Scattering
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Date
2017-06
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Addis Ababa University
Abstract
This study was initiated with the objectives to enhance the accuracy of determining the intended
components inside the selected milk samples like the amount of fat and protein contents and to
develop optical method for the determination of the amount of fat and protein content in the milk
samples. I implemented the ratio of the scattered to transmitted light intensity as the principal
analysis parameter to minimize the power fluctuation of the light source during doing the
experiment. The amount of fat contents and protein contents of five (Family, Etete, Harme,
Sholla and Mama) homogenized milk samples were determined. In this work we used laser light
scattering method. Finally, the data analysis was carried out using curve and surface fitting
method. My result shows that, the amount of fat contents in each (Family, Etete, Harme, Shola
and Mama) homogenized milk samples are 2.700%, 2.500%, 2.800%, 2.900% and 2.800%
respectively. But the amount of fat content written in the packets to each (Family, Etete, Harme,
Shola, Mama) milk samples are 2.700%, 2.800%, 2.800%, 2.700% and 2.700% respectively
which are almost consistent to experimental results. The amount of protein contents that is found
in each (Family, Etete, Harme, Shola and Mama) homogenized milk samples become 3.600 0
0 ,
3.900 0
0 , 3.800 0
0 , 3.500 0
0 and 3.500 0
0 respectively. The amount of protein contents written in
the packets to each (Family, Etete, Harme, Shola, Mama) milk samples are also 3.500%, 3.500%,
3.500%, 3.500% and 3.500% respectively which are also almost consistent to experimental
results. It may be concluded that, the laser light scattering method provides a simple and direct
method to determine fat and protein contents in investigated milk samples
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Keywords
Milk using He-Ne Laser Light Scattering