THE PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF SALMONELLA SPECIES FROM EXOTIC CHICKEN EGGS IN ALAGE AND SURROUNDING TOWNS, ETHIOPIA
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2014-06
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Abstract
This study was carried out from November 2012 to April 2013 to determine the prevalence and antibiotic susceptibility patterns of Salmonellaisolated from exotic chicken egg from Alage, Ziway and Shashemene farms. Two samples, from shell and content were taken from a single egg. Then pre-enrichment with buffered peptone water, enrichment with RV broth, plating on XLD and BGA agar were done. After that, positive samples transferred in to nutrient agar and biochemical tests (TSI agar, urea broth, lysine decarboxylation and indole test were done. From 196 eggs a total of 392 samples were taken and 52 (13.3%) were found to be positive of Salmonella. From these 52 positive samples 30 (7.7%) were from egg shell and 22 (5.6%) were from egg content.The total prevalence of Salmonella varied among the farming systems. The prevalence of Salmonellain egg content from semi-intensive farm (10%) was significantly higher than the prevalence of Salmonellafrom intensive farm (3.4%). The prevalence of Salmonella in egg shell from semi-intensive farm (11.5%) was also significantly higher than the prevalence of Salmonella in egg content from intensive farm (5.7%).The difference in prevalence observed between egg shell (7.7%) and egg content (5.6%) was having statistically significant difference.All the 52 Salmonella isolates were subjected to antimicrobial susceptibility test, using elevendifferent antimicrobials. Of these only one isolate was resistant to Ciprofloxacin and Ceftriaxon. Ampicillin is highly resisted (55.8%) followed by Tetracycline (36.5%), Naldixic acid (30.8%) and Sulphamethoxin-trimethoprim (30.8%).
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Antimicrobial Susceptibility, Egg content, Egg shell, Prevalence, Salmonella.