Spectrum of Fungal Pathogens in Sputum Samples from Pulmonary Tuberculosis Suspected Patients at Kotebe Primary Hospital Addis Ababa Ethiopia
| dc.contributor.advisor | Bitew Adane | |
| dc.contributor.author | Muluneh Tamagn | |
| dc.date.accessioned | 2018-12-13T07:52:59Z | |
| dc.date.accessioned | 2023-11-06T08:56:23Z | |
| dc.date.available | 2018-12-13T07:52:59Z | |
| dc.date.available | 2023-11-06T08:56:23Z | |
| dc.date.issued | 2018-10 | |
| dc.description.abstract | Background: pulmonary mycosis is a systemic fungal infection that occurs when fungi causeāsdiseases of the lungs. Mycobacterium tuberculosis (MTB), the causative agent of pulmonarytuberculosis is one of the major micro organisms that infect this site. Clinical and radiologicalcharacteristics of pulmonary mycosis are very similar to that of pulmonary tuberculosis therebymaking the disease easily misdiagnosed and mistreated as tuberculosis. Objective: To assess spectrum of fungal etiologies in sputum samples from pulmonarytuberculosis suspected patients at Kotebe primary hospital Addis Ababa, Ethiopia. Methods: A cross sectional descriptive study was conducted at Kotebe primary hospital on 423sputum samples from presumptive TB patients. Sputum samples were collected aseptically andPresence of pulmonary Tuberculosis was screened by Xpert MTB-Rif/assay. Microscopicallyfungal elements were screened using 10%KOH. Cultural identification of the differentpathogenic fungi was performed using SDA and BHI medium incubated at 25c0 and 37c0 for 4-6weeks respectively for each sputum samples. Identification was performed by noting; thegrowth form, and rate of growth, surface and reversed coloration on SDA and BHI agar plates.Yeasts were identified by conventional biochemical tests and assimilation characteristics. Dataanalysis was carried out using SPSS version 23 software. Results: 423 presumptive TB patients were enrolled in this study of which 50.6% were males.MTB was detected in 7.3% of the patients. Mycotic agents were isolated from 179(42.3%)patients of which 106(59.2%) were males. Co- infection of pulmonary Tuberculosis and a fungalagent was seen in 51.6% of MTB positive subjects. 62.7% of HIV positive patients includedshowed positive fungal culture. Out of the total isolates in this study, the genuses Aspergillusand Candida were the predominant fungal agents 8.7% and 23.9% respectively. C. cruzi was thepredominant fungi in MTB positive subjects. Other fungal etiologies isolated in our studyinclude; Penicillium, S. apiospermum, Fusarium, Mucor, Acremonium, Rhodotorulla, curvularia,Exserohilum species Conclusion; High incidence of fungal isolation (42.3%) was obtained in patients with respiratorysymptoms. We recommend for policy makers to conduct further studies and consider the need offungal screening in these patients | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/15070 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Respiratory fungal infection, presumptive TB patients, HIV infection | en_US |
| dc.title | Spectrum of Fungal Pathogens in Sputum Samples from Pulmonary Tuberculosis Suspected Patients at Kotebe Primary Hospital Addis Ababa Ethiopia | en_US |
| dc.type | Thesis | en_US |