Bacterial Profile And Antimicrobial Susceptibility Pattern Of Blood Culture Isolates At Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia
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Date
2017-03
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Addis Ababa University
Abstract
Back ground: Bloodstream infections are major health problems that lead to morbidity andmortality of patients unless treated with appropriate antimicrobial treatment. Drug resistance ofbacterial pathogens is challenging to alleviate sepsis. Blood culture is a gold standard techniquewhich provides essential information for the diagnosis and proper medication.
Objective: The Aim of study was to assess bacterial profile and antimicrobial susceptibilitypattern of blood culture isolates at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia
Method: A cross-sectional study was conducted from April 2016 to September 2016. Usingconvenient sampling technique a total of 422 blood sample were collected from study participant who were suspected for blood stream infection. Bacterial isolates were identified by colonymorphology, gram staining reaction, biochemical tests such as catalase, coagulase, optochin,PYR, triple sugar iron agar (TSI), citrate utilization, urease, motility, Indole were used forbacterial identification and susceptibility to antibiotics was done using Kirby Bauer discdiffusion technique method according to CLSI guidelines in Microbiology Laboratory of TikurAnbessa Specialized Hospital. The data were analyzed by using SPSS version 20.
Result: About 66(15.6%) microbial were isolated. Out of this 64 (96.9%) were bacterial and2(3.03%) were fungal (Candida albicans) isolates. The predominant bacterial isolates wereS.aureus (22.72%) and K.pneumoniae (16.66%). Gram positive cocci were highly resistance toPenicillin (86.7%), followed by Ceftriaxone (46.7%), and 40% were MRSA.While clindamycin(80 %), erythromycin (73.3%), and Trimethoprim-Sulfamethoxazole (66.7%) were effectiveantibiotics. Most of gram negative rods were highly resistance to ampicillin (85.7%), amoxicillinclavulanic acid (77.14%), Ceftriaxone (75%), and Ceftazidimen (62.8%). Nevertheless amikacin(97.1%), meropenem (91.5%), and ciprofloxacin (77.1%) were sensitive antibiotic. Most ofbacterial isolates (73.4%) were multidrug resistance to most frequently used antibiotics.
Conclusion: Overall, there was a high prevalence of blood stream infection with high resistancerate to the most commonly used antibiotics. Hence, timely investigation of blood streaminfection and regular surveillance of antimicrobial resistance pattern is important to reducemorbidity and mortality due to blood stream infection.
Keywords: Blood stream infection, Bacterial profile, Antimicrobial sensitivity pattern
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Keywords
Blood stream infection Bacterial profile Antimicrobial sensitivity pattern