Genetic Diversity Study of Anchote (Coccinia Abyssinica (Lam.) Cogn.) Using Inter Simple Sequence Repeat (Issr) Markers
| dc.contributor.advisor | Feyissa, Tileye (PhD) | |
| dc.contributor.author | Bekele, Abreham | |
| dc.date.accessioned | 2020-10-21T07:15:51Z | |
| dc.date.accessioned | 2023-11-18T09:50:15Z | |
| dc.date.available | 2020-10-21T07:15:51Z | |
| dc.date.available | 2023-11-18T09:50:15Z | |
| dc.date.issued | 2012-12-12 | |
| dc.description.abstract | Anchote (Coccinia abyssinica) is perennial trailing vine, underutilized but very important endemic plant with high calcium content grown for its edible tuberous roots in Ethiopia. In spite of its importance as food security crop, there is no information on molecular genetic diversity of this crop. Therefore, the objective of this study was to assess genetic diversity within and among 12 populations of anchote using ISSR markers. A total of 87 scorable bands were generated using nine ISSR primers among which 74 were polymorphic. Within population diversity based on polymorphic bands ranged from 13.8% to 43.53% with mean of 33.05%, Nei’s genetic diversity of 0.04 to 0.156 with mean of 0.118, Shannon information index of 0.07 to 0.23 with mean of 0.175 and AMOVA within population 51.4%. With all diversity parameters, the highest diversity was obtained from Gimbi, Bedele and Ale, whilst the lowest was from Manna. AMOVA showed 48.56% among populations variability and significantly lower than that of within population variation. Population differentiation with FST was 48.6%, impling high differentiation among population. From Jaccard’s pairwise similarity coefficient, Decha and Nedjo were most related populations exhibiting 0.76 similarity and Manna and Nedjo were the most distantly related populations with similarity of 0.52. The penta nucleotide primer 880 (GGAGA)3 showed unique band in some individuals that appeared to be associated with morphological traits. Illubabor and Gimbi populations exhibited highest genetic diversity so that the populations should be considered as the primary sites in designing conservation strategy and improvement of this crop. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/22882 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Anchote | en_US |
| dc.subject | Endemic | en_US |
| dc.subject | Genetic Diversity | en_US |
| dc.subject | ISSR | en_US |
| dc.subject | Underutilized | en_US |
| dc.title | Genetic Diversity Study of Anchote (Coccinia Abyssinica (Lam.) Cogn.) Using Inter Simple Sequence Repeat (Issr) Markers | en_US |
| dc.type | Thesis | en_US |