Genetic Diversity Study of Anchote (Coccinia Abyssinica (Lam.) Cogn.) Using Inter Simple Sequence Repeat (Issr) Markers
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Date
2012-12-12
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Addis Ababa University
Abstract
Anchote (Coccinia abyssinica) is perennial trailing vine, underutilized but very important
endemic plant with high calcium content grown for its edible tuberous roots in Ethiopia.
In spite of its importance as food security crop, there is no information on molecular
genetic diversity of this crop. Therefore, the objective of this study was to assess genetic
diversity within and among 12 populations of anchote using ISSR markers. A total of 87
scorable bands were generated using nine ISSR primers among which 74 were
polymorphic. Within population diversity based on polymorphic bands ranged from
13.8% to 43.53% with mean of 33.05%, Nei’s genetic diversity of 0.04 to 0.156 with
mean of 0.118, Shannon information index of 0.07 to 0.23 with mean of 0.175 and
AMOVA within population 51.4%. With all diversity parameters, the highest diversity
was obtained from Gimbi, Bedele and Ale, whilst the lowest was from Manna. AMOVA
showed 48.56% among populations variability and significantly lower than that of within
population variation. Population differentiation with FST was 48.6%, impling high
differentiation among population. From Jaccard’s pairwise similarity coefficient, Decha
and Nedjo were most related populations exhibiting 0.76 similarity and Manna and
Nedjo were the most distantly related populations with similarity of 0.52. The penta
nucleotide primer 880 (GGAGA)3 showed unique band in some individuals that
appeared to be associated with morphological traits. Illubabor and Gimbi populations
exhibited highest genetic diversity so that the populations should be considered as the
primary sites in designing conservation strategy and improvement of this crop.
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Keywords
Anchote, Endemic, Genetic Diversity, ISSR, Underutilized