Evaluation of Erythrocyte Sedimentation Rate Measurement by Iria Automated Method and Westergren Manual Method at Myungsung Christian Medical Center, Addis Ababa, Ethiopia
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Date
2017-06
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Addis Ababa University
Abstract
Background: - Erythrocyte sedimentation rate (ESR) is still a widely used hematologicalparameter as indicator of disease condition. Westergren method is the standard method based onInternational committee for Standardization in Hematology (ICSH) recommendation formeasuring ESR. But nowadays different automated instruments are available with reducedturnaround time, which increase the quality by reducing bio-hazard risk for the laboratoryprofessionals. However, there is no method comparison study conducted in Ethiopia and still theold method is used in many health facilities.
Objective: -Evaluation of Erythrocyte Sedimentation Rate measurement by IRIA automatedmethod and Westergren manual method at Myungsung Christian Medical Center, Addis Ababa.
Method: -A prospective cross sectional study was conducted at Myungsung Christian MedicalCenter from February to May 2017 GC. By doubling the ISCH recommendation the sampleswere tested in 3 different groups of values and the total sample size was 240. Every ESR orderedsample was analyzed using both manual and automated methods. Result was entered andanalyzed using SPSS version 20. Linear regression, correlation and Bland Altman analysis werecarried out to determine agreement between the reference and the new method was carried out.Agreement was considered acceptable when the difference is lying between mean + two standarddeviation (Mean+1.96SD) for 95% and above of cases in the Bland and Altman plot.
Result: The comparison between the conventional Westergren and IRIA automated methodshowed very good correlation in the two methods and agree well for ESR level between 1-20mm/hr with r=0.934, Mean bias 0.663, SD 2.629,LoA(5.815, -4.490), and ESR level between 21-60 mm/hr r=0.917, Mean bias 0.1875, SD 3.7924 LoA (7.621,-7.246). However, for ESR levelgreater than 60, it showed good correlation r=0.890 but poor agreement in the Bland-Altmananalysis as the mean difference is lying between the limit of agreement for less than 95% ofcases Mean difference 1.388, SD 11.346,LoA (23.625,-20.849).
Conclusion: For ESR level below 60 mm/hr, the two instruments can be used interchangeably.However, for ESR level greater than 60mm/hr the result of the automated machine must beconfirmed with the conventional westergren method before using it for clinical propose.
Key words: - Erythrocyte Sedimentation Rate, Westergren, IRIA, ESR automated
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Keywords
Erythrocyte Sedimentation Rate, Westergren, IRIA, ESR automated