Simultaneous Determination of Dopamine and Pyridine-2- aldoxime methochloride at Poly (3, 4-ethylenedioxythiophene) Modified Glassy Carbon Electrode
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Date
2016-03
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Addis Ababa University
Abstract
A sensitive and selective method was developed for the simultaneous determination of
Dopamine (DA) and Pyridne-2-alidoxime methochiloride (PAM-2) with poly (3, 4-
ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode (GCE). Cyclic
voltammetry and Square wave adsorptive stripping voltammetry (SWAdSV) were used to
investigate the electrochemical reaction of DA and PAM-2 at the PEDOT modified electrode.
DA and PAM-2 were showed a diffusion control and adsorption control reaction mechanisms,
in phosphate buffer solution of pH 7.0 respectively. The significant anodic peak potential
difference (525 mV) between DA and PAM-2 enabled the simultaneous determination of both
species based on Square wave adsorptive stripping voltammetry. The voltammetric responses
gave linear ranges of 5×10 −7 –1.0×10 −4 mol L −1 and 5.0×10 −6 –15.0×10 -5 molL −1 , with detection
limits of 3.1×10 −8 molL −1 and 1.91×10 −7 molL −1 for DA and PAM-2, and The method was
successfully applied for the determination of DA and PAM-2 in biological samples, with in a
very good recovery result 101.57 % and 95.17 % respectively.
Keywords: Dopamine (DA), Pyridine-2-aldoxime methochloride (PAM-2), Glassy carbon
electrode (GCE), Poly (3, 4-ethylenedioxythiophene) (PEDOT), Cyclic voltammetry (CV) and
Square wave adsorptive stripping voltammetry (SWAdSV)
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Keywords
Dopamine (DA); Pyridine-2-aldoxime methochloride (PAM-2); Glassy carbon electrode (GCE); Poly (3; 4-ethylenedioxythiophene) (PEDOT); Cyclic voltammetry (CV) and Square wave adsorptive stripping voltammetry (SWAdSV)