Establishment of reference intervals for the common renal and liver function clinical chemistry parameters among apparently healthy pregnant and non-pregnant women in South wollo zone, Amhara National Regional State, northeast Ethiopia.
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Date
2019-11
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Addis Ababa University
Abstract
Background: Physiological changes during pregnancy causes alterations in biochemical
analytes concentration. Thus, pregnancy specific reference intervals (RI) are important for
accurate diagnosis and treatment of cases. In Ethiopia, clinical laboratory test results are
usually interpreted using values established on western population.
Objective: To establish RIs for common renal and liver function clinical chemistry
parameters among apparently healthy pregnant and non- pregnant women in South wollo
zone, Amhara National Regional State, northeast Ethiopia.
Methods: A community based cross sectional study was conducted on a total of 378
apparently healthy study participants randomly selected from south wollo zone, Ethiopia
from April to June 2019. Data like medical history, physical examination and sociodemography
were collected by using well organized questionnaire. After the exclusion of
outliers using quartile method, Kolmogorov–Sminorv test was used to check its normality.
The 95% RI with 95% confidence interval was established using the non-parametric
method. The significance of differences between pregnant and non-pregnant women was
evaluated using Mann–Whitney U test.
Result: There was statistically significant variation between pregnant and non-pregnant
women in values of Albumin, T.Protein, ALP, urea and Creatinine but not for AST, ALT,
bilirubin(direct) and bilirubin(total). Reference intervals established for pregnant women
includes: albumin 26.14–42.87g/l, total protein 48.52–74.71 g/l, AST 2.4–43.6 U/L, ALT
0.94–28.35 U/L, ALP 21.2–337 U/L, bilirubin(direct) 0.03-0.32 mg/dl, bilirubin(total) 0.26-
0.94 mg/dl, creatinine 0.29–0.87 mg/dl, urea 7.17–20.82 mg/dl. Albumin: 32.81–47.87,
total protein: 56.71–83.9 U/L, AST: 4.2–37.1 U/L, ALT: 2.69–41.18 U/L, ALP: 3.22–278.7
U/L, bilirubin(direct) 0.1-0.51mg/dl, bilirubin(total) 0.24-1.06mg/dl, creatinine 0.44–1.00
mg/dl, urea 8.07–27.87 mg/dl for non-pregnant women.
Conclusion: The RIs provided by this study are decisive in interpreting clinical laboratory
results for medical decision making and other health-related conditions. Physiological
adaptations of pregnancy should be taken into account when interpreting LFT and RFT in a
pregnant woman.
Description
This is to certify that the thesis prepared by Miftah MohammedAssen, entitled: Establishment of reference
intervals for the common renal and liver function clinical chemistry parameters among apparently
healthy pregnant and non-pregnant women in South wollo zone, Amhara National Regional State,
northeast Ethiopia submitted in partial fulfillment of the requirements for Master of Science degree in
Clinical Laboratory Sciences (Clinical Chemistry track) complies with the regulations of the University and
meets the accepted standards with respect to originality and quality.
Keywords
Reference interval, Clinical chemistry, Biochemical analytes, South wollo, Healthy, pregnant, non-pregnant