Establishment of Community based Reference Interval for Common Clinical Chemistry Parameters among Adolescents and Children in Addis Ababa, Ethiopia

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Date

2020-06

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Addis Abeba University

Abstract

Back ground: Establishment of Reference Intervals (RI) is one of the expected activities of medical laboratories which enable interpretation of the laboratory results according to the existing local setups. Company derived values are being used to interpret results in health facilities of Addis Ababa by the absence of locally RI. Objective: To establish RI for common clinical chemistry parameters among apparently healthy adolescents and children in Addis Ababa, Ethiopia from April to October, 2019 Methods: A community based cross sectional study was conducted on a total of 516 apparently healthy children (5-11 years) and adolescents (12-17 years) randomly selected from Addis Ababa, Ethiopia from April to October, 2019.After interviewing the health status of participants, socio demographic, nutritional status and life style data were collected using structured questionnaire. Blood (5 ml), urine (5ml) and stool (about 5 grams) samples was taken and examined. Serum levels of selected clinical chemistry parameters were determined using Cobas C501. Data were entered, cleaned and analyzed by SPSS version 21. After the exclusion of outliers using turkey method, Kolmogorov-Sminorv test was used to check its normality. The 95% RI with 90% CI was determined using non-parametric method (2.5 and 97.5 th percentile).The difference between children and Adolescent values was evaluated using Mann-Whitney test and P-value of <0.05 claimed the presences of stastically significant difference. Results: There was stastically significant variation between children and Adolescents in both sex and age for level of AST, ALP, TP, TBIL, DBIL, Cr, and UA, but ALT and ALB by age and Urea by sex. The established RI includes: Gluc 61-91 and 57-97 mg/dl, ALT 1.3-13.0 and 1.6-14.0 U/L, AST 12.3-26.7 and 8.0-27.0U/L, ALP 143-375 and 47-452U/L, DBIL 0.01-0.20 and 0.01-0.27mg/dl, TBIL 0.09-0.46 and 0.16-0.71mg/dl, TP6.4-7.8 and 6.48.3g/dl, ALB 4.1-5.1 and 4.1-5.2g/dl, UA1.4-4.9 and 1.5-6.8mg/dl, Cr 0.29-0.58 and 0.040.64mg/dl and Urea 9-30 and 8-31mg/dl for children and Adolescents, respectively for each test. Conclusion: The study identified variations with the currently utilized RIs; therefore, establishment and use of local reference ranges should be encouraged because it enhances patient care and health research. th

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Keywords

References interval, Clinical chemistry, Biochemistry analytes, Apparently Healthy, Adolescent, children

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