Establishment of Community based Reference Interval for Common Clinical Chemistry Parameters among Adolescents and Children in Addis Ababa, Ethiopia
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Date
2020-06
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Journal Title
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Addis Abeba University
Abstract
Back ground: Establishment of Reference Intervals (RI) is one of the expected activities of
medical laboratories which enable interpretation of the laboratory results according to the
existing local setups. Company derived values are being used to interpret results in health
facilities of Addis Ababa by the absence of locally RI.
Objective: To establish RI for common clinical chemistry parameters among apparently
healthy adolescents and children in Addis Ababa, Ethiopia from April to October, 2019
Methods: A community based cross sectional study was conducted on a total of 516
apparently healthy children (5-11 years) and adolescents (12-17 years) randomly selected
from Addis Ababa, Ethiopia from April to October, 2019.After interviewing the health status
of participants, socio demographic, nutritional status and life style data were collected using
structured questionnaire. Blood (5 ml), urine (5ml) and stool (about 5 grams) samples was
taken and examined. Serum levels of selected clinical chemistry parameters were determined
using Cobas C501. Data were entered, cleaned and analyzed by SPSS version 21. After the
exclusion of outliers using turkey method, Kolmogorov-Sminorv test was used to check its
normality. The 95% RI with 90% CI was determined using non-parametric method (2.5
and
97.5
th
percentile).The difference between children and Adolescent values was evaluated
using Mann-Whitney test and P-value of <0.05 claimed the presences of stastically
significant difference.
Results: There was stastically significant variation between children and Adolescents in both
sex and age for level of AST, ALP, TP, TBIL, DBIL, Cr, and UA, but ALT and ALB by age
and Urea by sex. The established RI includes: Gluc 61-91 and 57-97 mg/dl, ALT 1.3-13.0
and 1.6-14.0 U/L, AST 12.3-26.7 and 8.0-27.0U/L, ALP 143-375 and 47-452U/L, DBIL
0.01-0.20 and 0.01-0.27mg/dl, TBIL 0.09-0.46 and 0.16-0.71mg/dl, TP6.4-7.8 and 6.48.3g/dl,
ALB 4.1-5.1 and 4.1-5.2g/dl, UA1.4-4.9 and 1.5-6.8mg/dl, Cr 0.29-0.58 and 0.040.64mg/dl
and
Urea
9-30
and
8-31mg/dl
for
children and Adolescents, respectively for each
test.
Conclusion: The study identified variations with the currently utilized RIs; therefore,
establishment and use of local reference ranges should be encouraged because it enhances
patient care and health research.
th
Description
Keywords
References interval, Clinical chemistry, Biochemistry analytes, Apparently Healthy, Adolescent, children