Production of Starch Based Antimicrobial Packaging Films with Optimal Physical and Mechanical property
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Date
2015-03
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Addis Ababa University
Abstract
The aim of this work was to build up starch-based bioactive films from corn starch, glycerol, citric
acid, and cinnamon bark oleoresin and characterize its physical and mechanical properties, and
check its antimicrobial effect. The processing variables were optimized to study their effect on the
functional properties of bio- films. Glycerol concentration (20, 30, & 40%v/w), citric acid
concentration (0, 5, & 10%w/w), and drying temperature (30, 60, & 90oc) as independent variables
which produced 20 different combinations, were studied using response surface methodology to
investigate the effect of these variables on the product responses. The glycerol concentration was the
most influential parameter for the physical and mechanical properties of the prepared packaging
films followed by citric acid concentration and drying temperature. The numerical optimization gave
in glycerol concentration (27.86ml glycerol/100g corn starch), citric acid concentration (0.29g citric
acid/100g corn starch), and drying temperature (62.84oc) as optimum variables to produce better
films. Using the optimized variables the films were re-prepared and the functional groups were
identified using FTIR. The bio-films were again prepared using the optimized variables with the
addition of Cinnamon bark oleoresin (0%, 10%, and 12% v/w of dry starch) in order to analyze its
antimicrobial activities against the target microorganisms Escherichia coli, Staphylococcus aureus
and Pseudomonas aeruginosa. The cinnamon bark oleoresin showed clear inhibition zone against
the tested microorganisms whereas the bio-films containing the oleoresin showed surface inhibition.
Keywords: Antimicrobial activities, Starch based bioactive films, Target microorganisms
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Keywords
Antimicrobial Activities, Starch Based Bioactive Films, Target Microorganisms