Determination of Common Hematological Parameters Reference Intervals among Apparently Healthy Pregnant and Non-Pregnant women of South Wollo Zone, Amhara region, Northeast Ethiopia.
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Date
2019-12
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Addis Ababa University
Abstract
Background: The physiology of a normal pregnancy involves major alterations in thehematological parameters. However, hematological parameters reference intervals currentlybeing used in Ethiopia is derived from western populations, even though significant variationsare reported previously.
Objective: To determine hematological parameters reference intervals among apparently healthypregnant and non-pregnant women of South wollo zone, Amhara region, Northeast Ethiopia.
Method: A community based cross-sectional study was conducted among 600 pregnant and nonpregnantwomen in South Wollo Zone, Amhara Region, Northeast Ethiopia from June to August2019. Medical history, physical examination and laboratory screening were performed for eachstudy participant. Socio-demographic and other important data were collected using a structured questionnaire. 4ml of Whole blood was collected and Mindary BC-3000 plus hematologyanalyzer was used to measure hematological parameters following standard procedures. Datawere entered and analyzed using SPSS version 23. The mean, median, and 2.5th percentile and97.5th reference intervals limits with 90% CI were determined for the hematological parameters.P-value <0.05 was considered statistically significant.
Result: The established reference intervals (2.5th–97.5th percentile) for pregnant women were:WBC: 4.0-13.2x103/ul; Lymph %: 12.9-38.13 %; Gran %: 50.45-81.54 %; MID %: 4.24-11.64;RBC: 3.45-4.67x106/ul; Hgb: 10.1-13.67 g/dl; HCT: 33.49-46.52 %; MCV: 84.76-103.52 fl;MCH: 27.5-33.0 pg; Platelet count: 131.7-373.15x103/ul; MPV: 7.24- 10.16 fl. A statisticalsignificant difference between pregnant and non-pregnant was noted in all hematologicalparameters except MCHC. The mean and median value of WBC count, absolute granulocytecount and percentage, MCV, MPV and PDW increased, whereas mean values of HCT andPlatelet count decreased as gestational period advance.
Conclusion: The findings of this study indicated that pregnant women hematological parametersreference intervals differ from other studies in Ethiopian and other countries pregnant womenreference intervals. Hence, this would signify the necessity for such determinations of localreference intervals for different populations and the health facilities found in south wollo zoneshould utilized the currently established reference intervals for pregnant women for better care.
Description
This is to certify that the thesis prepared by Mesfin Fiseha, entitled: Determination ofHematological Parameters Reference Intervals among apparently healthy pregnant andnon-pregnant women of south wollo zone, Amhara region, Northeast Ethiopia andsubmitted in partial fulfillment of the requirements for Master of Science degree in ClinicalLaboratory Sciences (Hematology and Immunohematology) complies with the regulations of the
University and meets the accepted standards with respect to originality and quality.
Keywords
Reference interval, Hematological parameter, pregnant women, trimester, South Wollo