Determination of Common Hematological Parameters Reference Intervals among Apparently Healthy Pregnant and Non-Pregnant women of South Wollo Zone, Amhara region, Northeast Ethiopia.
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Date
2019-12
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Addis Ababa University
Abstract
Background: The physiology of a normal pregnancy involves major alterations in the
hematological parameters. However, hematological parameters reference intervals currently
being used in Ethiopia is derived from western populations, even though significant variations
are reported previously.
Objective: To determine hematological parameters reference intervals among apparently healthy
pregnant and non-pregnant women of South wollo zone, Amhara region, Northeast Ethiopia.
Method: A community based cross-sectional study was conducted among 600 pregnant and nonpregnant
women in South Wollo Zone, Amhara Region, Northeast Ethiopia from June to August
2019. Medical history, physical examination and laboratory screening were performed for each
study participant. Socio-demographic and other important data were collected using a structured
questionnaire. 4ml of Whole blood was collected and Mindary BC-3000 plus hematology
analyzer was used to measure hematological parameters following standard procedures. Data
were entered and analyzed using SPSS version 23. The mean, median, and 2.5th percentile and
97.5th reference intervals limits with 90% CI were determined for the hematological parameters.
P-value <0.05 was considered statistically significant.
Result: The established reference intervals (2.5th–97.5th percentile) for pregnant women were:
WBC: 4.0-13.2x103/ul; Lymph %: 12.9-38.13 %; Gran %: 50.45-81.54 %; MID %: 4.24-11.64;
RBC: 3.45-4.67x106/ul; Hgb: 10.1-13.67 g/dl; HCT: 33.49-46.52 %; MCV: 84.76-103.52 fl;
MCH: 27.5-33.0 pg; Platelet count: 131.7-373.15x103/ul; MPV: 7.24- 10.16 fl. A statistical
significant difference between pregnant and non-pregnant was noted in all hematological
parameters except MCHC. The mean and median value of WBC count, absolute granulocyte
count and percentage, MCV, MPV and PDW increased, whereas mean values of HCT and
Platelet count decreased as gestational period advance.
Conclusion: The findings of this study indicated that pregnant women hematological parameters
reference intervals differ from other studies in Ethiopian and other countries pregnant women
reference intervals. Hence, this would signify the necessity for such determinations of local
reference intervals for different populations and the health facilities found in south wollo zone
should utilized the currently established reference intervals for pregnant women for better care.
Description
This is to certify that the thesis prepared by Mesfin Fiseha, entitled: Determination of
Hematological Parameters Reference Intervals among apparently healthy pregnant and
non-pregnant women of south wollo zone, Amhara region, Northeast Ethiopia and
submitted in partial fulfillment of the requirements for Master of Science degree in Clinical
Laboratory Sciences (Hematology and Immunohematology) complies with the regulations of the
University and meets the accepted standards with respect to originality and quality.
Keywords
Reference interval, Hematological parameter, pregnant women, trimester, South Wollo