Study on Prevalence of Mastitis and Associated Risk Factors with Isolation and Anitimicrobial Susceptibility of Major Pathogs in Alage State Dairy farm, Ethiopia
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Date
2012-06
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Addis Ababauniversity
Abstract
Cross-sectional study design was implemented in Alage dairy farm from September 2011 to April 2012 to determine the overall prevalence of mastitis, isolation of mastitis pathogens with their anti-biogram susceptibility patterns and risk factors to mastitis were also investigated. 444 quarter of 111 exotic dairy cows were examined. Overall prevalence of mastitis at cow and quarter level was found to be 73% and 37% respectively. Among 444 quarters examined 23(5.2%) were blind leaving 421 quarters functional. Sub clinical mastitis was diagnosed by using California Mastitis Test reagent. Different bio- chemical tests were used to identify bacteria and the highest prevalent bacteria was found to be Coagulase negative staphylococci (CNS) (37.7%) followed by Staphylococcus aureus (19.6 %), Escherichia coli (9.4%), Staphylococcus intermidius (9.4%), Bacillus species (8%), Streptococcus species (5.8%), Klebsiella pneumoniae (5.8%), and Enterobacter aerogens (4.3%). Among seven anti-biotics tested in vitro most isolates were sensitive to Norfloxacin but showed resistance to Ampicillin. Age, stage of lactation, milk yield, hygiene and feet problems and udder conformation were found to be risk factors to mastitis with significant difference P<0.05. However, parity, blind teat and body condition score showed non-significant difference (P>0.05). The high prevalence rate of mastitis in this study implied that, it is the trickiest health problem of dairy cows of the study area. Isolation of both environmental and contagious bacteria revealed unhygienic milking procedure. Therefore, milkers should be trained on proper hygienic milking methods, regular investigation of mastitis especially sub clinical form should be practiced, mastitis treatments should be preceded with identification of the causative agent and susceptibility test profile of pathogens and culling of old aged and repeatedly infected cows should be done on regular planned basis.
Key words: Alage, anti-biotics, bacterial isolates, clinical, dairy, exotic, mastitis, prevalence, risk factors, sub clinical
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Keywords
Alage, anti-biotics, bacteria, isolates, clinical, dairy, exotic, mastitis, prevalence, risk factors, sub clinical