Evaluation of acute and sub-chronic Toxicity of Aqueous Leaves Extracts of Maytenus Gracilipes Celastraciae (kombolcha) on Some Blood Parameters and Histopathology of Liver and kidney in Swiss Albino Mice
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Date
2015-08
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Addis Abeba University
Abstract
Human beings use plants for the purpose of disease control and prevention. Maytenus gracilipes
celastraciae is a plant having vital traditional medicinal values for treating several human
ailments such as for headache, epilepsy, allergic, peptic ulcer, cancer and immflamations. The
present study was carried out to evaluate the acute and subchronic toxic effects of aqueous leaves
extracts of maytenus gracilipes celastraciae (kombolcha) on some blood parameters and
histopathology of liver and kidney in mice. LD50 was determined.
The thesis was carried out in Addis Abeba University, College of Health Science, School of
Medicine, Department of Anatomy (Histology Laboratory).The study was conducted from May,
2014 -July, 2015.The aqueous leaves extracts of M.gracilipes were employed in swiss albino
mice in single oral dose for acute study and repeated oral dose for subchronic study.
The LD50, of aqueous extracts of Maytenus gracilipes was found to be 10,000 mg/kg body
weight. In the Subchronic study, the extract was administered orally at doses of 700 and
2100 mg/kg/day for 90 days . Body weight, biochemical and hematological parameters
were determined at the end of the 90 days of daily extract administration.
In sub chronic toxicity study daily oral administration of aqueous extract at 700 and 2100 mg/kg
body weight/day did not result
in death or significant
changes
in body weight,
hematological and biochemical parameters. Studies on Histopathological examination of
selected organs (liver and kidney) showed normal architecture suggesting absence of
morphological disturbances.
However, at 700 mg/kg body weight liver showed pyknotic nuclei and at 2100mg/kg some
cellular infiltrates near the central vein and portal region, where as, in kidney peritubular
infiltration and focal mono nuclear leukocytic infiltration was observed at higher dose.
Key words: Maytenus gracilipes, Aqueous extract, acute toxicity, subchronic toxicity,
Histopathological studies.
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Keywords
Maytenus Gracilipes; Aqueous Extract; Acute Toxicity; Subchronic Toxicity; Histopathological studies