Isolation and Characterization of the 34 Kd Gene of Mycobacterium Leprae.
| dc.contributor.advisor | W.M., Peter (PhD) | |
| dc.contributor.author | Abebe, Feseha | |
| dc.date.accessioned | 2018-06-28T12:26:25Z | |
| dc.date.accessioned | 2023-11-08T16:39:41Z | |
| dc.date.available | 2018-06-28T12:26:25Z | |
| dc.date.available | 2023-11-08T16:39:41Z | |
| dc.date.issued | 1993-09 | |
| dc.description.abstract | An M. Iprae::pHC79 cosmid genomic library was screened for the identification, isolation and characterization of the 34 kd antigen gene of Mycobacterium leprae using its homologue, the 34 kd antigen gene of Mycobacterium tuberculosis, as a DNA probe. Qut of a total of 79 different cosmids screened, covering about 88 % of the genome of M. leprae, one cosmid designated as BL 1527 was found to harbor the gene of interest. Restriction enzyme analysis of the cosmid BL 1527 yielded four DNA fragments - two containing the complete gene and two containing part of the gene. The four DNA segments were subcloned in plasmid vectors pUCBM21 and pBKS+ and characterized physically and immunologically. Physical characterization of the gene by nucleotide sequencing revealed that the 34 kd antigen gene has an open reading frame of 801 nucleotides encoding 266 amino acid residues bearing a molecular weight of28,887 daltons and an isoelectric point of 4.3. Homology comparison with the 34 kd antigen gene from M.tuberculosis has revealed 80.6 % (633 nucleotides) identity at the DNA level and 85 % (221 amino acids) identity at the protein level. Immunological characterization by western blot analysis has shown that the gene is expressed in E. coli from its own promotor signals and that the recombinant protein is recognized by the monoclonal antibody F-126. 2 which is specific for the mycobacterial 34 kd antigens. Crossed Immuno-electrophoresis analysis has established that the recombinant 34 kd antigen corresponds to the non secreted Antigen 84 of mycobacteria. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/4684 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | Isolation and Characterization of the 34 Kd Gene of Mycobacterium Leprae. | en_US |
| dc.type | Thesis | en_US |