Determination of Hematological Parameters Reference Interval for Apparently Healthy Population aged 15 to 60 years in Addis Ababa, Ethiopia
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Date
2021-07
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Addis Abeba University
Abstract
Background: Complete blood count (CBC) reference intervals were essential for effectively
diagnosing diseases, measuring drug toxicity or side effects, disease staging and monitoring of
response to treatment, therapeutic management decision, or other physiological assessment in the
clinical laboratory and assessing overall health. They may also be used in clinical trials as a
guide to set inclusion and exclusion criteria. Factors such as genetics, dietary patterns, gender,
age, ethnic origin, altitude, pregnancy, and geographical factors and geochemical and
environmental pathogens were known to influence hematological reference intervals (RIs).
Hence, there is a need to determine locally relevant hematological reference intervals.
Objectives: To determine hematological parameters reference intervals for apparently healthy
population aged 15 to 60 years in Addis Ababa.
Methods: A cross- sectional community based study consisting 790 males and females (pregnant
and non-pregnant) at household level was conducted from January to May 2019 in Addis Ababa,
Ethiopia.. A complete blood count (CBC) was performed using Sysmex XT1800i automated
hematology analyzer. The non-parametric test as per the CLSI guide was used to determine the
2.5th and 97.5th percentiles. SPSS-IBM version 24 statistical software was used for data entry
and analysis. Differences among groups were tested and level of significanc was set at P value
less than 0.05.
Result: Males comprise 262, and 346 non-pregnant females and 182 pregnant women were
included. The 2.5
th
and 97.5
th
RIs determined were somehow inconsistent with the RI currently
in use. RBC count, Hgb, HCT and MCHC were significantly higher in males than females while
median value for MCV, MCH, RDW-CV and RDW-SD values were higher in females than
males. Non-pregnant females had significantly higher median PLT count and PCT than males
and pregnant women. Pregnant women had significantly higher median PDW, MPV, P-LCR
than their non-pregnant counterparts and males. A decrease in RBC parameters and increase in
WBC count was observed with increasing trimester.
Conclusion: As differences were noted with the company derived RIs and other population
groups, the current RI needs to be in use in Addis Ababa.
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Keywords
Reference Interval, Hematological parameters, Complete blood count, Ethiopia