Determination of Hematological Parameters Reference Interval for Apparently Healthy Population aged 15 to 60 years in Addis Ababa, Ethiopia

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Addis Abeba University


Background: Complete blood count (CBC) reference intervals were essential for effectively diagnosing diseases, measuring drug toxicity or side effects, disease staging and monitoring of response to treatment, therapeutic management decision, or other physiological assessment in the clinical laboratory and assessing overall health. They may also be used in clinical trials as a guide to set inclusion and exclusion criteria. Factors such as genetics, dietary patterns, gender, age, ethnic origin, altitude, pregnancy, and geographical factors and geochemical and environmental pathogens were known to influence hematological reference intervals (RIs). Hence, there is a need to determine locally relevant hematological reference intervals. Objectives: To determine hematological parameters reference intervals for apparently healthy population aged 15 to 60 years in Addis Ababa. Methods: A cross- sectional community based study consisting 790 males and females (pregnant and non-pregnant) at household level was conducted from January to May 2019 in Addis Ababa, Ethiopia.. A complete blood count (CBC) was performed using Sysmex XT1800i automated hematology analyzer. The non-parametric test as per the CLSI guide was used to determine the 2.5th and 97.5th percentiles. SPSS-IBM version 24 statistical software was used for data entry and analysis. Differences among groups were tested and level of significanc was set at P value less than 0.05. Result: Males comprise 262, and 346 non-pregnant females and 182 pregnant women were included. The 2.5 th and 97.5 th RIs determined were somehow inconsistent with the RI currently in use. RBC count, Hgb, HCT and MCHC were significantly higher in males than females while median value for MCV, MCH, RDW-CV and RDW-SD values were higher in females than males. Non-pregnant females had significantly higher median PLT count and PCT than males and pregnant women. Pregnant women had significantly higher median PDW, MPV, P-LCR than their non-pregnant counterparts and males. A decrease in RBC parameters and increase in WBC count was observed with increasing trimester. Conclusion: As differences were noted with the company derived RIs and other population groups, the current RI needs to be in use in Addis Ababa.



Reference Interval, Hematological parameters, Complete blood count, Ethiopia