Genetic diversity of Plasmodium falciparum field isolates based on two PCR markers Merozoite Surface Protein 1 and 2 from kolla-shelle area, Arbaminch Zuria district, South West Ethiopia

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Addis Ababa University


Background: The population structure of the causative agents of human malaria, Plasmodium species including the most serious agent Plasmodium falciparum ( P.falciparum), depends on the local epidemiological and demographic situations, such as the incidence of infected people, the vector transmission intensity and migration of inhabitants (i.e. exchange between sites).One of the major characteristics of malaria parasites is their genetic diversity and an increasing number of studies have been reported on the population structure variation of P. falciparum based on the polymorphism of merozoite surface protein (MSP) 1 and 2. Limited data however are available from Ethiopia. Objective: To evaluate the extent of genetic diversity of P falciparum in Kola-Shele in South West of Ethiopia. Methods: Health facility based cross sectional study design was employed to determine the prevalence of genetic diversity of P.falciparum in Kola-Shele area. Eighty-eight stored dried blood spot samples which were collected between September and December, 2008 were used. Parasite DNA was extracted from the blood spot on to filter paper and analyzed by length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions targeted block 2 of msp-1 and block 3 of msp-2,including their allelic families: K1,MAD20,RO33 and FC27,3D7/IC1, respectively. Data entry was done using Microsoft Excel sheet and was double entered to verify accuracy; data was analyzed using SPSS for windows 16 soft ware (SPSS INC, Chicago, IL, USA). Results: The total number of alleles identified in MSP1 block 2 was 11, while 12 alleles were observed in MSP2 block 3.In MSP1, K1was found to be the predominant allelic type, carried alone, with MAD20 and RO33 type. In MSP2, 3D7/IC was the most identified. Forty- three and sixty nine percent of isolates MSP1 and MSP2, respectively had high multiple genotypes and the overall mean multiplicity of infection was 1.8 (95% CI: 1.48-2.04). Conclusion: The Genetic diversity in P. falciparum field isolates in kolla-Shelle area were mixed and multiple infections were observed. K1 and 3D7/IC1 were the most predominant circulating allelic families



Arbaminch Zuria district