In Vitro Propagation of Korarima (Aframomum Corrorima) From Shoot Explants
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Date
2016-09
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Addis Ababa University
Abstract
This study with a title of in vitro propagation of Aframomum corrorima from shoot explant is done by Teferi Mekonen at Addis Ababa University, inistitute of biotechenology. Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is herbaceous perennial plant that belongs to the family Zingiberaceae. It is an indigenous spice crop to Ethiopia. Lack of a steady supply of quality planting material is one of the bottlenecks in the production of this crop for local consumption and export. Therefore, the objective of this study is to develop protocol for in vitro propagation of A. corrorima, by using shoot tip as explant for mass propagation. Shoot tips were cultured in MS medium supplemented with Thidiazuron (TDZ) in combination with α-Naphthalene acetic acid (NAA) or Indol-3-acetic acid (IBA) to investigate their effect on shoot multiplication. The multiple clums of shoots were then separated as individual shoot and well developed shoots transferred in to 1/2 MS medium supplemented with NAA, IAA and IBA for root induction. Statistical analysis revealed that there was significant difference among treatments applied in both shoot multiplication and rooting experiments. There was also 100% initiation of shoot after first week of culture in all treatment of shoot multiplication. Average Maximum numbers of shoots per explant (2.97 ± 1.43) were obtained on MS medium containing 0.5 mg/l of TDZ in combination with 0.25 mg/l IBA. The highest numbers of mean roots per shoot (7.13 ± 5.21) was obtained on medium containing 1.0 mg/l of NAA and 0.5 mg/l of IAA combinations. After acclimatization, 86% plants survived in greenhouse. Key words: Growth regulators, Rooting, Shoot initiation, Shoot multiplication,
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Growth regulators, Rooting, Shoot initiation, Shoot multiplication