In Vitro Regeneration of Sweet Potato (Ipomoea Batatas (L.) Lam.) From Leaf and Petiole Explants
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Date
2011-10
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Addis Ababa University
Abstract
Sweet potato (Ipomoea batatas (L.) Lam.) is a tuber-bearing species and it is an economically important crop in tropical, sub-tropical and warm temperate regions. It is a staple root crop serving as a source of vitamins, proteins and carbohydrate. The establishment of in vitro plant regeneration system in sweet potato is of potential importance to sweet potato quality improvement and genetic transformation. The study was conducted to develop in vitro protocol for use in plant regeneration from leaf and petiole explants of Beletech and Awassa-83 sweet potato varieties through callus. For this study, twelve different combinations of 2,4-D and kinetin were used for callus induction from petiole and leaf explants of Beletech and Awassa-83 sweet potato varieties on MS medium. Among the entire combinations, the medium supplemented with 0.05 mg/l 2,4-D in combination with 0.5 mg/l kinetin resulted in maximum percentage of calli formed (90 and 83) from leaf and petiole explants of Beletech variety whereas 93% and 87% from petiole and leaf explants of Awassa-83 variety, respectively. Among different concentrations of BAP used for shoot regeneration, maximum percentage (80 and 77) of shoot regeneration were obtained from leaf calli of Beletech and Awassa-83 whereas 70% and 71% were obtained from petiole calli of Beletech and Awassa-83 varieties, respectively, on media without growth regulators. BAP in combination with GA3 was found to be less efficient for shoot multiplication than BAP alone for shoots regenerated from leaf and petiole originated calli of the two sweet potato varieties. Among the different concentrations of BAP used for shoot multiplication, 1.0 mg/l resulted in maximum of two shoots per node from shoots regenerated from petiole and leaf calli of both varieties. Maximum shoot heights recorded were 11.10 cm, 11.40 cm for shoots regenerated from Beletech petiole and leaf calli, and 10.70 cm and 11.90 cm for shoots regenerated from petiole and leaf calli of Awassa-83 varieties, respectively. Variety difference, concentration of IBA and explant difference significantly affected the average number of main root and root length of the two varieties. Among the different concentrations of IBA used for rooting, 0.00 mg/l was the best with main roots 8.10, 6.80 per shoot and root length 10.00 cm and 10.10 cm for shoots regenerated from petiole and leaf calli of Beletech variety, respectively. However, for Awassa-83, 0.1 mg/l IBA was the most suitable concentration of IBA with mean number of roots 6.07 and 4.83 per shoot and mean length of 10.40 cm and 8.70 cm for shoots regenerated from petiole and leaf calli, respectively. Among all plantlets planted in the glasshouse, 90 and 100% for shoots regenerated from petiole and leaf calli of Beletech variety and 80 % and 90% for shoots regenerated from petiole and leaf calli of Awassa-83 variety, respectively were survived.
Key words/Phrases: Awassa-83，Beletech, Rooting，Shoot Multiplication
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Keywords
Awassa-83, Beletech, Rooting, Shoot Multiplication