A one health assessment of antimicrobial use and associated resistance in Escherichia coli along the beef value chain in Akaki Kality sub city, Addis Ababa, Ethiopia
| dc.contributor.advisor | Takele Beyene | |
| dc.contributor.author | Chaltu Desalegn | |
| dc.date.accessioned | 2026-07-03T13:16:30Z | |
| dc.date.available | 2026-07-03T13:16:30Z | |
| dc.date.issued | 2026 | |
| dc.description.abstract | Improper use of antimicrobials (AMU) in food animals contributes the emergence of antibiotic resistant bacteria, which subsequently disseminate through the food chain between animals and humans, posing significant threats to public health and food security. This study aimed to assess AMU and antimicrobial resistance (AMR) profile of Escherichia coli (E. coli) and identify potential risk factors for resistance along the beef value chain in Akaki Kality sub city, Addis Ababa. A cross-sectional study design was employed from November 2025 to May 2026. Data collection included laboratory analysis, farmers‟ knowledge, attitudes, and practices (KAP) regarding AMU and AMR, and assessment of hygienic practice among meat handlers. Statistical analysis of Fisher‟s exact test was used to analyze KAP data, while Chi-square and logistic regression for laboratory findings. This study showed that, overall 50%, 46.2% and 42.3% respondents have poor knowledge, attitude, and practice toward AMU and AMR, respectively. Low educational level, small farm size, shorter experience in beef fattening, limited training and lack of access to AMU and AMR information were risk factor of poor KAP of farmers. Of 663 samples examined, 176 (26.5%) were positive for E. coli and 19(2.9%) for E. coli O157: H7. E. coli isolates were recovered from beef farms 31(18.7%), abattoir 88(34.2%), and butcher shops 57(23.8%), while E. coli O157:H7 isolates were detected in farms 7(4.2%), abattoir 9(3.5%), and butcher shops 3(1.3%). Antimicrobial susceptibility testing against 12 antibiotics revealed that E. coli isolates were most susceptible to chloramphenicol 16(69.6%), imipenem 13(56.5%), and ciprofloxacin 13(56.5%), whereas E. coli O157:H7 isolates were most susceptible to, imipenem 13(68.4%), chloramphenicol 12(63.2%) and amoxicillin clavulanate 11(57.9%). Multidrug resistance was observed in 17(73.9%) E. coli and 16 (84.2%) E. coli O157:H7 isolates. In general, the study revealed challenges in proper AMU, hygiene practices, and high prevalence of microbial contamination along the beef value chain. These findings collectively emphasize the need for integrated One Health interventions addressing antimicrobial stewardship, farm biosecurity, meat hygiene, and AMR surveillance. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/8603 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Abeba University | |
| dc.subject | Antimicrobial resistance | |
| dc.subject | Antimicrobial use | |
| dc.subject | Beef value chain | |
| dc.subject | Escherichia coli | |
| dc.subject | Food safety | |
| dc.subject | One Health | |
| dc.title | A one health assessment of antimicrobial use and associated resistance in Escherichia coli along the beef value chain in Akaki Kality sub city, Addis Ababa, Ethiopia | |
| dc.type | Thesis |