Prevalence of Pathogenic Bacteria and Antimicrobial Susceptibility Patterns among Patients with Ear infection at Arsho Advanced Medical Laboratory, Addis Ababa Ethiopia.
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Date
2019-11
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Addis Ababa University
Abstract
Background: Otitis media is a major health problem and occurs with a high incidence andprevalence in both developed and developing countries. The burden and populationdemographics of otitis media differ greatly between developed and developing regions. SubSaharan Africa account for most deaths from complications arising from otitis media.
Objective: The objective of this study was to determine the bacterial isolates and their drugsusceptibility patterns from patients who had ear infection at Arsho Advanced MedicalLaboratory.
Methods: Cross-sectional study was conducted at Arsho medical laboratory, Addis Ababa,Ethiopia from January to April 2019. By using an aseptic technique ear discharge samples werecollected by a sterile swab and inoculated on Blood Agar, Chocolate Agar and Mac Conkey'smedia. Isolated bacterial species were identified and drug susceptibility testing was performed byusing the Vitek 2 system (bio-Mérieux). Data were entered and analyzed by using SPSS version20 software and P-value of < 0.05 was considered statistically significant.
Results: Out of 422 samples processed, bacterial pathogens were isolated from19.5% (82) samples. From the total bacterial isolated 78% were gram negative bacteria and thepredominant isolate was Pseudomonas species and Proteus spps 21.9 % each followed byKlebsiella spps 20.3%. Among gram negative bacteria isolates 46.9 % had multiple antibioticresistant patterns. Pseudomonas aeruginosa 92.4% and proteus vulgaris had 80 % wereresistant against the commonly used antibiotics Ampicillin & Amoxicillin.
Conclusion: Majority of the bacterial isolates were multidrug resistant, hence, efforts to isolatemicroorganisms and determine the susceptibility pattern should be strengthened to improve thetreatment outcome of otitis media instead of the usual trend of empirical treatment.
Description
This is to certify that the thesis prepared by Melesse Desse, entitled: Distribution of BacterialIsolates And their Drug Susceptibility of Ear Discharge among Patients with Ear InfectionReferred to Arsho Advanced Medical Laboratory and submitted in partial fulfillment of therequirements for Master of Science degree of postgraduate program in clinical laboratory science
(diagnostic and public health microbiology). Complies with the regulations of the University andmeets the accepted standards with respect to originality and quality
Keywords
Ear infection, Multidrug resistance and Antimicrobial susceptibility.