Prevalence of pathogenic bacteria and antimicrobial susceptibility patterns among patients with ear infection at Arsho Advanced Medical Laboratory, Addis Ababa Ethiopia.

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Addis Ababa University


Background: Otitis media is a major health problem and occurs with a high incidence and prevalence in both developed and developing countries. The burden and population demographics of otitis media differ greatly between developed and developing regions. Sub Saharan Africa account for most deaths from complications arising from otitis media. Objective: The objective of this study was to determine the bacterial isolates and their drug susceptibility patterns from patients who had ear infection at Arsho Advanced Medical Laboratory. Methods: Cross-sectional study was conducted at Arsho medical laboratory, Addis Ababa, Ethiopia from January to April 2019. By using an aseptic technique ear discharge samples were collected by a sterile swab and inoculated on Blood Agar, Chocolate Agar and Mac Conkey's media. Isolated bacterial species were identified and drug susceptibility testing was performed by using the Vitek 2 system (bio-Mérieux). Data were entered and analyzed by using SPSS version 20 software and P-value of < 0.05 was considered statistically significant. Results: Out of 422 samples processed, bacterial pathogens were isolated from 19.5% (82) samples. From the total bacterial isolated 78% were gram negative bacteria and the predominant isolate was Pseudomonas species and Proteus spps 21.9 % each followed by Klebsiella spps 20.3%. Among gram negative bacteria isolates 46.9 % had multiple antibiotic resistant patterns. Pseudomonas aeruginosa 92.4% and proteus vulgaris had 80 % were resistant against the commonly used antibiotics Ampicillin & Amoxicillin. Conclusion: Majority of the bacterial isolates were multidrug resistant, hence, efforts to isolate microorganisms and determine the susceptibility pattern should be strengthened to improve the treatment outcome of otitis media instead of the usual trend of empirical treatment.


This is to certify that the thesis prepared by Melesse Desse, entitled: Distribution of Bacterial Isolates And their Drug Susceptibility of Ear Discharge among Patients with Ear Infection Referred to Arsho Advanced Medical Laboratory and submitted in partial fulfillment of the requirements for Master of Science degree of postgraduate program in clinical laboratory science (diagnostic and public health microbiology). Complies with the regulations of the University and meets the accepted standards with respect to originality and quality


Ear infection, Multidrug resistance and Antimicrobial susceptibility.