Micropropagation of Andrachne Aspera Spreng From Shoot Tip and Nodal Segments
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Date
2019-09-09
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Addis Ababa University
Abstract
Andrachne aspera belongs to Euphorbiacea family and mostly grows in dray woody land or bush land and in dry sandy or stony cover bed at elevation from sea level up to (2000m-2400m). This plant is mostly used as antibiotic and to treat poison of snake and malaria. The seeds were washed under running tap water followed by washing in 70% ethanol for 2 to 3minutes. These seeds were surface sterilized using 20% Clorox for 10 minute and washed using sterile distilled water. The sterilized seeds were then cultured on growth regulator free Murashige and Skoog (MS) medium. After germination, shoot tips were excised and cultured on MS medium supplemented with different concentrations BAP or kinetin for culture initiation. The initiated shoot tips were transferred to solid MS medium supplemented with different concentrations of BAP or kinetin in combination with NAA. On shoot initiation medium, MS medium containing 1.5 mg/l BAP resulted in highest mean shoot number per explants (5.03±0.29). On shoot multiplication medium, the highest mean shoot number per explants (5.12±0.29 from shoot tip), 5.23±0.22 from node explants and highest mean shot length (4.30±0.20 cm) were obtained on medium containing 0.5mg/l BAP in combination with 0.25 mg/l NAA. The highest mean number of roots per shoot (6.00± 0.77) and mean root length (6.00±0.55 cm) were obtained on half strength MS medium containing 0.25 mg/l IAA. After acclimatization, 90% plants survived in green house. BAP (1.5mg/l) was best for shoot induction and BAP in combined with NAA(0.5mg/l BAP +0.25 NAA) produced best result for shoot multiplication. Rooting of microshoots was successful in 0.25 mg/l of IAA. Cultivation of the propagated plant to provided medicine and reduce the endanger status of the plant.
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Keywords
Acclimatization, Andrachne Aspera, Growth Regulators, In Vitro Propagation, Shoot Multiplication, Rooting