Study on mycobacterium bovis using conventional and molecular methods in cattle slaughtered in Kombolcha Elfora Meat processing plant
| dc.contributor.advisor | Dr. Yilkal Asfaw, Dr. Gobena Ameni, | |
| dc.contributor.author | Fekadu, Desta | |
| dc.date.accessioned | 2020-06-17T09:58:14Z | |
| dc.date.accessioned | 2023-11-30T12:54:18Z | |
| dc.date.available | 2020-06-17T09:58:14Z | |
| dc.date.available | 2023-11-30T12:54:18Z | |
| dc.date.issued | 2008-06 | |
| dc.description.abstract | A cross-sectional study was conducted on 1138 heads of cattle at Kombolcha ELFORA meat processing plant, South-Wello Administrative Zone, Amarha Regional State from November 2007 to May 2008. The objectives of the study were to estimate the prevalence of bovine tuberculosis in cattle slaughtered at Kombolcha ELFORA meat processing plant, and to isolate and characterize the strains of mycobacteria from tuberculousis suspicious lesions. Detailed post mortem examination, bacteriological culturing, regions of difference-bases polymerase chain reaction and spoligotyping were used. The prevalence of bovine tuberculosis was 5.0 % (57/1138) in cattle slaughtered at Kombolcha ELFORA meat processing plant on the basis of detailed post-mortem examination. There was no significance difference in prevalence between male and female (P > 0.05), as well as among the age groups (P > 0.05). Mycobacteria were able to be isolated from 28 of the 57 cattle with gross lesion. A total of 49 isolates were recovered from the different tissues of these 28 cattle, and 20 isolates showed signal for M. tuberculosis complex species of which 19 were M. bovis while one isolate was M. tuberculosis. Further characterization of the strains of M. bovis using spoligotyping revealed the presence of five different cluster of spoligotyping pattern, which include Ethiopian M. bovis train 1, SB1176, SB0134, SB0133 and new strain. The binary number representation of the new strain is 1100000101010110111111111111111101111100000 where 1 indicates the presence of a spacer and 0 indicates a loss. The new spoligotyping was not reported previously from elsewhere to the M. bovis database (www.mbovis.org). The findings of this study indicated that tuberculous lesions were common in cattle slaughtered at Kombolcha ELFORA meat processing plant, and hence detailed post mortem meat inspection procedures are required to minimize the risk of its transmission to the public through meat consumption. In addition, appropriate cooking of meat is required before consumption. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/21664 | |
| dc.language.iso | en | en_US |
| dc.subject | Bovine tuberculousis | en_US |
| dc.subject | Mycobacterium bovis | en_US |
| dc.subject | Pathology scoring | en_US |
| dc.subject | Molecular typing | en_US |
| dc.title | Study on mycobacterium bovis using conventional and molecular methods in cattle slaughtered in Kombolcha Elfora Meat processing plant | en_US |
| dc.type | Thesis | en_US |