Study on mycobacterium bovis using conventional and molecular methods in cattle slaughtered in Kombolcha Elfora Meat processing plant
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2008-06
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Abstract
A cross-sectional study was conducted on 1138 heads of cattle at Kombolcha ELFORA meat
processing plant, South-Wello Administrative Zone, Amarha Regional State from November
2007 to May 2008. The objectives of the study were to estimate the prevalence of bovine
tuberculosis in cattle slaughtered at Kombolcha ELFORA meat processing plant, and to
isolate and characterize the strains of mycobacteria from tuberculousis suspicious lesions.
Detailed post mortem examination, bacteriological culturing, regions of difference-bases
polymerase chain reaction and spoligotyping were used. The prevalence of bovine
tuberculosis was 5.0 % (57/1138) in cattle slaughtered at Kombolcha ELFORA meat
processing plant on the basis of detailed post-mortem examination. There was no significance
difference in prevalence between male and female (P > 0.05), as well as among the age
groups (P > 0.05). Mycobacteria were able to be isolated from 28 of the 57 cattle with gross
lesion. A total of 49 isolates were recovered from the different tissues of these 28 cattle, and
20 isolates showed signal for M. tuberculosis complex species of which 19 were M. bovis
while one isolate was M. tuberculosis. Further characterization of the strains of M. bovis
using spoligotyping revealed the presence of five different cluster of spoligotyping pattern,
which include Ethiopian M. bovis train 1, SB1176, SB0134, SB0133 and new strain. The
binary number representation of the new strain is
1100000101010110111111111111111101111100000 where 1 indicates the presence of a
spacer and 0 indicates a loss. The new spoligotyping was not reported previously from
elsewhere to the M. bovis database (www.mbovis.org). The findings of this study indicated
that tuberculous lesions were common in cattle slaughtered at Kombolcha ELFORA meat
processing plant, and hence detailed post mortem meat inspection procedures are required to
minimize the risk of its transmission to the public through meat consumption. In addition,
appropriate cooking of meat is required before consumption.
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Keywords
Bovine tuberculousis, Mycobacterium bovis, Pathology scoring, Molecular typing