Phytochemial Investigations and Biological Activities of Smoke Samples Collected from Otostegia Integrifolia, Silene Macrosolen, and Echinops Kebericho Plants
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Date
2020-08-06
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Addis Ababa University
Abstract
The essential oils and smoke collected from leaves of Otostegia integrifolia, S. macrosolen and E.kebericho were investigated using gas chromatography mass spectrometry (GC-MS). Several compounds were identified in the essential oil of Otostegia integrifolia: They were Terpenoids (51.51%) which are monoterpene (12.12%), sesqui terpene (36.36% ), di terpene (3.03%) and phenolic compounds ( 13.60% ). From smoke trapped in hexane a group of compound such as phenolic (43.73%), alkane (21.88% ), terpene (9.3%) were identified. In smoke trapped by methanol, alkenes (62.50%), and phenolic compounds were detected. From smoke of S. macrosolen trapped in hexane and methanol phenolic compounds 54.00% and 70.37% respectively were identified. From smoke of E.kebericho trapped in hexane and methanol phenolic compound 57.89% and 65.20% respectively were identified. The antioxidant activities were evaluated using reducing power (FRAP), by UV-Vis absorption spectroscopy. The results revealed that the Silene macrosolon exhibited the highest anti-oxidant activity compared to Echinops kebercho and Otostegia integrifolia. The antioxidant concentration in Hexane trapped smoke of all sample under this study was negligible. The insecticidal activity tests (both repellent and mortality) were conducted using cockroach as a test insect. The repellant activity of insect was tested by O. integrifolia leaves extract and, the recorded results of repellency at 60 minutes were 100% by hexane and 91.47% by methanol trapped smokes. The maximum mortality of cockroach at the maximum time by E. kebericho, S. macrosolen and O. integrifolia smoke extract were 100.00 , 40.00 , 13.2% respectively.
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Essential Oil, Otostegia Integrifolia, Silene Macrosolon, Echinops Kebericho and Antioxidant, Mortality Rate