Bacterial Profile and Drug Susceptibility Patterns of Isolates from Surgical Site Infections, Healthcare workers’ Hands and the Surrounding Environment at Surgical wards of Saint Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia
| dc.contributor.advisor | Desta, Kassu (Ass. Prof.) | |
| dc.contributor.advisor | Asmamaw, Asegedech (PhD fellow) | |
| dc.contributor.advisor | Gebreyohannes, Zenebe | |
| dc.contributor.author | Melaku, Gelila | |
| dc.date.accessioned | 2026-01-30T11:49:13Z | |
| dc.date.available | 2026-01-30T11:49:13Z | |
| dc.date.issued | 2025-10 | |
| dc.description.abstract | Background: Surgical site infections (SSIs) are infections affecting wounds from invasive surgery. Hospitals harbor many bacteria, including antibiotic-resistant strains, on frequently touched surfaces like work areas, medical equipment, and even furniture, which pose a significant risk for the development of SSIs. Objective: To assess the bacterial profile and drug susceptibility patterns of isolates from SSIs, health care workers’(HCWs’) hands and the surrounding environment at surgical wards of Saint Paul's Hospital Millennium Medical College (SPHMMC) Addis Ababa, Ethiopia. Methods: A cross-sectional study, was conducted from June to October, 2024, and collected a total of 327 samples. All samples were inoculated on MacConkey and Blood agar. Gram staining and biochemical assays were used for identification of the bacteria. Antimicrobial susceptibility testing was done on Muller-Hinton agar by using the Kirby-Bauer disk diffusion technique and data analysis, was performed using SPSS version 26. Results: The study revealed that out of total 327 samples 90.2 % were harboring bacterial growth. Only 9.8 % samples showed no bacterial growth. Mixed bacterial growth seen in 22.4%. A total of 368 bacterial isolates were identified. Gram-positive bacteria comprised 57.6 % (212) of the isolates, while Gram-negative bacteria accounted for the remaining 42.4% (156). High resistance rates were observed among Gram-negative isolates, including ceftriaxone resistant rate of 91.1%.Whereas, Gram-positive isolates showed high resistance against penicillin 89.7%. This study revealed a high prevalence of MDR, affecting 82.7% (177/214) of the isolates. Conclusion and recommendation: This study highlights a high prevalence of bacterial contamination in SSIs, HCWs' hands, and surgical ward environment. Klebsiella species, S.aureus, and E.coli were identified as the major contributors to postoperative SSIs. It exposes critical concerns regarding antibiotic resistance in both Gram-positive and Gram-negative bacteria. These findings emphasize the urgent need for effective infection control measures and antibiotic stewardship. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/7615 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Surgical site infection | |
| dc.subject | Health care worker | |
| dc.subject | Environmental sample | |
| dc.subject | Bacterial profile and drug resistance | |
| dc.title | Bacterial Profile and Drug Susceptibility Patterns of Isolates from Surgical Site Infections, Healthcare workers’ Hands and the Surrounding Environment at Surgical wards of Saint Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia | |
| dc.type | Thesis |