Establishing Umbilical Cord Blood Hematological Parameters Reference Interval for Newborns in St. Peter Specialized Hospital Addis Ababa, Ethiopia from January 1 to March 31, 2019
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Date
2019-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Abeba University
Abstract
Background: Several factors like altitude, age, sex, pregnancy, socioeconomic status, life style
and race influence hematological reference interval (RIs), which are critical to support appropriate
clinical decisions and to interpret laboratory data in research. Currently there are no wellestablished
RIs
for
cord
blood
hematological
parameters
of
newborns
in Ethiopia.
Objective:
To
generate reference interval for umbilical cord blood hematological parameters of
newborns at St Peter Specialized Hospital Addis Ababa, Ethiopia.
Method: A cross-sectional study was conducted from January 1 to March 31, 2019 on healthy,
term newborns (37-42 weeks) with normal birth weight born to apparently healthy pregnant
mothers who had met the eligibility criteria. From a total of 139 newborns, 2-3ml cord blood was
immediately collected from the clumped cord using EDTA tube. The samples were analyzed using
Sysmex KX 21 hematology analyzer. Data was entered and the 2.5
th
and 97.5
th
percentile (upper
and lower reference limit) were determined using non parametric method by SPSS version 23. The
non-parametric independent Mann-Whitney U test (Wilcoxon rank-sum test) was used to compare
the distribution of the parameters between genders, modes of deliveries and gestational age groups.
Result: The combined reference interval for umbilical cord blood hematological parameters of
newborns with the median and 95% reference values were as follows for WBC= 12.4 [6.55-19.35],
RBC= 4.51 [3.55-5.52], HGB= 15.80 [12.41-19.65], HCT= 45.9[37.9-56.3], MCV=102.10[83.90111.55],
MCH= 35.30 [29.35-39.10], MCHC= 34.3 [32.3-37.40], PLT= 236 [146-438], LYM=
37.5% [16.6-63.0%], MXD= 7.9%[1.65-15.75%], NEU= 53.7%[30.3-78.3], RDW= 15.6[12.019.0],
PDW=11.0[9.1-15.7] and MPV= 9.4[8.1-11.8]. The current study found no significance
difference between genders, except RDW (P=0.01) and gestational age group, but there was
significance difference for WBC (p=0.007), RBC (p=0.018) and Absolute NEU (p=0.001).
Conclusion: The values obtained from our study provide reference intervals for some
hematological parameters in healthy newborns of Addis Ababa and its surrounding special
weredas. However, the results need to be confirmed by larger samples from different centers
throughout the country.
Description
Keywords
CBC, Reference interval, Umbilical Cord Blood, Neonates