Establishing Umbilical Cord Blood Hematological Parameters Reference Interval for Newborns in St. Peter Specialized Hospital Addis Ababa, Ethiopia from January 1 to March 31, 2019
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Date
2019-07
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Addis Abeba University
Abstract
Background: Several factors like altitude, age, sex, pregnancy, socioeconomic status, life style and race influence hematological reference interval (RIs), which are critical to support appropriateclinical decisions and to interpret laboratory data in research. Currently there are no wellestablishedRIs forcordblood hematologicalparameters of newborns in Ethiopia.
Objective:Togenerate reference interval for umbilical cord blood hematological parameters ofnewborns at St Peter Specialized Hospital Addis Ababa, Ethiopia.
Method: A cross-sectional study was conducted from January 1 to March 31, 2019 on healthy,term newborns (37-42 weeks) with normal birth weight born to apparently healthy pregnantmothers who had met the eligibility criteria. From a total of 139 newborns, 2-3ml cord blood wasimmediately collected from the clumped cord using EDTA tube. The samples were analyzed usingSysmex KX 21 hematology analyzer. Data was entered and the 2.5thand 97.5th percentile (upperand lower reference limit) were determined using non parametric method by SPSS version 23. The non-parametric independent Mann-Whitney U test (Wilcoxon rank-sum test) was used to comparethe distribution of the parameters between genders, modes of deliveries and gestational age groups.
Result: The combined reference interval for umbilical cord blood hematological parameters ofnewborns with the median and 95% reference values were as follows for WBC= 12.4 [6.55-19.35],RBC= 4.51 [3.55-5.52], HGB= 15.80 [12.41-19.65], HCT= 45.9[37.9-56.3],MCV=102.10[83.90111.55],MCH= 35.30 [29.35-39.10], MCHC= 34.3 [32.3-37.40], PLT= 236 [146-438], LYM=37.5% [16.6-63.0%], MXD= 7.9%[1.65-15.75%], NEU= 53.7%[30.3-78.3], RDW= 15.6[12.019.0],PDW=11.0[9.1-15.7] and MPV= 9.4[8.1-11.8]. The current study found no significance difference between genders, except RDW (P=0.01) and gestational age group, but there was significance difference for WBC (p=0.007), RBC (p=0.018) and Absolute NEU (p=0.001).
Conclusion: The values obtained from our study provide reference intervals for somehematological parameters in healthy newborns of Addis Ababa and its surrounding specialweredas. However, the results need to be confirmed by larger samples from different centersthroughout the country.
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Keywords
CBC, Reference interval, Umbilical Cord Blood, Neonates