Establishment of community based Hematological Reference Intervals among apparently healthy adolescents aged 12-17 years in Mekelle city, Tigrai, Northern Ethiopia; a Cross Sectional Study Design From December, 2018 – May, 2019

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Addis Ababa University


Background: Hematological reference ranges are important in clinical and diagnostic management for the assessment of health and disease conditions. Hematological reference intervals are better to be established based on sex and age differences as these are among the main factors affecting them. Objective: The aim of this study was to establish hematological reference intervals among adolescents aged, 12-17 years in Mekelle, Tigrai, and Northern Ethiopia, 2019. Study area: conducted in Mekelle city Study design and period: using a cross sectional study design conducted from December 2018 to May 2019 G.C. Method: A community-based cross-sectional study was conducted in 249 adolescents aged 12-17 years from December 2018 to May 2019. About 4ml of blood sample was collected using vacutainer tube containing di-potassium Ethylenediamine tetra-acetic acid. Hematological parameters were measured by Sysmex KX-21N hematology analyzer (Sysmex Corporation Kobe, Japan). The data were entered and analyzed by SPSS version 23 statistical software. The 97.5th percentile and 2.5th percentile were the upper and lower reference limit for the study population. Result: A total of 249 adolescents consisting of 122 (49%) males and 127 (51%) females with the median age of 15.31 (range 12 to 17) years were recruited. The median, mean and 95% percentile ranges of hematological values were determined. The 95% RI values were: Red blood cells (1012/Liter), 4.6-5.9 (Males) and 4.28-5.75 (Females); White blood cells (109/Liter), 2.9-9.6 (Males) and 3.4-10.2 (Females); Hemoglobin (g/dl), 12.6-17.6 (Males) and 12-15.4 (Females); Platelets(109/Liter), 138-364 (Males) and 150.6-461.8 (Females). Almost all of the hematological parameters showed significant differences (p<0.05) across the gender. Conclusion: This study concluded that there were statistically significant higher values of WBC count, MCV, platelet count, lymphocyte count, and neutrophil percent in females than males. However the value of RBC count, hemoglobin, hematocrit, MCHC, RDW CV (%), mixed count, and mixed percentage were statistically significant higher in males than females.



Adolescents, Apparently healthy, CBC, Ethiopia, Hematological parameters, Reference interval