Isolation and Molecular Characterization of Mycobacteria from Cattle Slaughtered at Adama Abattoir
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Date
2010-06-01
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Addis Ababa University
Abstract
Bovine tuberculosis (BTB) is endemic in Ethiopia. and the epidemiological picture of the disease
has not yet been established. Skin testing and abattoir-based detection of lesions are the main
tools for studying the epidemiology of BTB. A cros sectional study was conducted on 960 cattle
slaughtered at Adama abattoir in east Shoa zone of Oromia region to estimate its prevalence and
characterize its causative agents. Detail postmortem examination and patholog:- . coring.
bacteriological culturing. and multiplex polymerase chain reaction (PCR) were applied in this
study. On the basis of gross lesions. the prevalence of BTB was 12.7% (122 /960) in cattle
slaughtered at Adama abattoir. The caudal mediastinal lymph nodes constituted the most severely
affected tissue (mean± SEM; 0.55 ± 0.81) followed by cranial mediastinal (0. 308 ± 0.060). and
left bronchial (0.\33 ± 0.042) lymph nodes. Multivariable logistic regression analysis sho\wed
that cattle between 9-12 years of age were at a higher risk of infection (OR = 6.43: Cl = 2.43.
17.04) with TB. The result of genus typing of the eleven isolates using multiplex PCR indicated
seven non- mycobaclerium tuberculosis complex members and only one Mycobacterium
tuberculosis complex member while the remaining three isolates did not show signal neither for
thc Genus :mycobacterium nor for it species. In conclusion. the results of the present stud) have
indicated that TB lesions wcre prevalent in tissues of cattle slaughtered at Adama aballoir while
the predominant mycobacteria causing such lesions were mycobacteria which were not members
of Mycobacterium tuberculosis complex. And hence. further identification and characterization
of these mycobacteria is required.
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Keywords
Bovine tuberculosis. Culturing, Multiplex PCR. mycobacteriulI1 tubetrculosis• complex, Prevalence. TB lesion.