Quality assessment of platelet concentrates prepared from different time intervals held whole blood at Ethiopian Blood and Tissue Bank Service, Addis Ababa, Ethiopia.
No Thumbnail Available
Date
2023-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Background: The objective of this research is to meet the ever-growing demand for blood and blood
components in health facilities, especially platelet concentrate, which is extremely high. The short
shelf life of platelet concentrate is another reason for the shortage. Therefore, with the need to
generate a new additional method to increase the supply of platelet concentrate, this study uses
platelet concentrates prepared from whole blood held at different time intervals. In addition, it
included almost all quality assurance requirements to ensure its quality.
Objective: The purpose of this study was to assess quality of platelet concentrates prepared from
different time intervals held whole blood at Ethiopian Blood and Tissue Bank Service, Addis Ababa.
Methods: A Cross sectional research was done on 153 samples taken from 25 female and 128 male
non-ruminated volunteer blood donors and whose age was range 18 to 61 years at Ethiopian Blood
and Tissue Bank Service from August 1 to October 30, 2022. Convenient sampling was used; all
whole blood collected at mobile and static centers were included. The selected whole blood was held
for 8, 16 and 24 hours at room temperature and processed into its component according to SOPs;
then platelet concentrates were assessed for their quality parameters based on the national standards.
The data collection was checked before analytical, during analytical and after analytical steps then,
data was entered, checked, cleaned, and fed into Microsoft Excel software and then imported into
SPSS version 26 for further statistical analysis. Mann-Whitney U test, the Wilcoxon Signed-Rank
Test and Friedman‘s test were used for pairwise group comparison, within group pairwise
comparisons and triple measure within group median comparisons, respectively. P <0.05 was
statistically significant.
Results: In this study, swirling was observed in all units tested. Mean of volume were within the
range 50-70ml. Mean of platelet count of 8, 16 and 24hrs held prepared platelet concentrates were
above ≥5.5x1010/unit at day 1, 3 and 5. Mean of white blood cell count and pH level were in
acceptable range <0.2x109 and 6.4-7.4 respectively. The quality parameters of the 16 and 24 hours
storage held prepared platelet concentrates were comparable with those of the 8 hours. All
parameters except red blood cell count met the Ethiopian Blood and Tissue Bank Service‘s standard
requirement (75%). Statistically no significant difference were observed when compared to the
platelet concentrates quality indicators of three different time interval prepared platelet concentrates,
except volume and WBC count were have significant difference in comparison.
Conclusion: This study found that delaying whole blood processing for up to 16 and 24 hours had
no effect on platelet concentrates quality indicators when compared to 8 hours held produced platelet
concentrates.(p >0.05)
Description
Keywords
Whole blood, Platelet concentrate, platelet quality