Bacterial Profiles and Their Antimicrobial Susceptibility Patterns From Body Fluids at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia

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Addis Ababa University


Background: Body fluids serve as a medium for carrying nutrients and waste products. These fluids are usually sterile, even if a single colony of bacteria may be significant to invade and infect to body fluids and leads to morbidity and mortality. Assessing to the prevalence of bacteria and testing their antibiotic susceptibility helps to provide effective therapies, develop rational prescription writing and make policy decisions. Objective: The aim of this study was determining bacterial profiles and their antimicrobial susceptibility patterns from body fluids at Tikur Anbessa Specialized Hospital, laboratory Addis Ababa, Ethiopia. Method: A cross sectional study was conducted from july 2015 to September 2015 and 384 study participants were required. CSF, acsites, pleural and synovial fluid were collected and cultured on Blood agar, MacConkey agar and chocolate agar and incubated according to standard conditions. All specimens were subjected to Gram Stain, WBC count and AFB immediately following culturing. All culture positives were identified by gram stain and biochemical tests using the standard procedure and antimicrobial susceptibility test was performed using Kirby-Bauer method. Data was entered and analyzed using SPSS version 22. Result: In this study 54/384(14. 0%) of bacteria were isolated from different body fluids such as; Cerebrospinal fluids 264(68.8%), Pleural fluid 76(19.8%), Peritoneal fluid 34(8.9%), and Synovial fluid 10 (2.6%) and using gram stains 41/384 (10.7%) of bacteria were seen from the total of body fluids. Among these majority of body fluids 173(44.1%) had abnormal white blood cell count (WBC) above 05 cells per mm3, out of them 91(21.1%) had polymorphic feature. K. pneumonia 16.7 %( 9/32) were the most common isolated bacterial following by coagulase negative Staphylococcus 15.0 %( 8/32). Among all body fluid in CSF (31(57.4%) were the highest bacterial isolated. Out of 54 isolates, 41(75.9%) of bacteria were showing multi drug resistance. Gentamycin (76%) and erythromycin (59%) were the highest drug resistanc for gram-negative and gram-positive respectively. Conclusion: The prevalence of bacterial isolates in this study was 14.0%. Frequency of single as well as multiple drug resistance was high. Over all 41/54, (75.9%) isolates had shown Multiple-drug-resistance of (MDR≥2) drugs



Bacterial Profiles, Antimicrobial Susceptibility Patterns