Bacterial Profiles and Their Antimicrobial Susceptibility Patterns From Body Fluids at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia
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Date
2017-05
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Addis Ababa University
Abstract
Background: Body fluids serve as a medium for carrying nutrients and waste products.
These fluids are usually sterile, even if a single colony of bacteria may be significant to
invade and infect to body fluids and leads to morbidity and mortality. Assessing to the
prevalence of bacteria and testing their antibiotic susceptibility helps to provide effective
therapies, develop rational prescription writing and make policy decisions.
Objective: The aim of this study was determining bacterial profiles and their antimicrobial
susceptibility patterns from body fluids at Tikur Anbessa Specialized Hospital, laboratory
Addis Ababa, Ethiopia.
Method: A cross sectional study was conducted from july 2015 to September 2015 and 384
study participants were required. CSF, acsites, pleural and synovial fluid were collected and
cultured on Blood agar, MacConkey agar and chocolate agar and incubated according to
standard conditions. All specimens were subjected to Gram Stain, WBC count and AFB
immediately following culturing. All culture positives were identified by gram stain and
biochemical tests using the standard procedure and antimicrobial susceptibility test was
performed using Kirby-Bauer method. Data was entered and analyzed using SPSS version
22.
Result: In this study 54/384(14. 0%) of bacteria were isolated from different body fluids
such as; Cerebrospinal fluids 264(68.8%), Pleural fluid 76(19.8%), Peritoneal fluid
34(8.9%), and Synovial fluid 10 (2.6%) and using gram stains 41/384 (10.7%) of bacteria
were seen from the total of body fluids. Among these majority of body fluids 173(44.1%)
had abnormal white blood cell count (WBC) above 05 cells per mm3,
out of them
91(21.1%) had polymorphic feature. K. pneumonia 16.7 %( 9/32) were the most common
isolated bacterial following by coagulase negative Staphylococcus 15.0 %( 8/32). Among
all body fluid in CSF (31(57.4%) were the highest bacterial isolated. Out of 54 isolates,
41(75.9%) of bacteria were showing multi drug resistance.
Gentamycin (76%) and
erythromycin (59%) were the highest drug resistanc for gram-negative and gram-positive
respectively.
Conclusion: The prevalence of bacterial isolates in this study was 14.0%. Frequency of single
as well as multiple drug resistance was high. Over all 41/54, (75.9%) isolates had shown
Multiple-drug-resistance of (MDR≥2) drugs
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Keywords
Bacterial Profiles, Antimicrobial Susceptibility Patterns