Bio-Butanol Production from Napier Grass Hydrolysis Using Clostridium Acetobutylicum
| dc.contributor.advisor | Solomon, Kiros (PhD) | |
| dc.contributor.author | Biruk, Yohannes | |
| dc.date.accessioned | 2022-06-18T08:14:28Z | |
| dc.date.accessioned | 2023-11-28T13:30:27Z | |
| dc.date.available | 2022-06-18T08:14:28Z | |
| dc.date.available | 2023-11-28T13:30:27Z | |
| dc.date.issued | 2022-02 | |
| dc.description.abstract | Napier grass is a potential biomass for biofuel production due to its high adaptability and widespread availability in tropical areas such as Ethiopia. A compositional analysis of Napier grass collected from the Menagesha Mushroom farm in an Addis Ababa city suburb was performed, and microbes and enzymes were purchased from France. An alkaline pretreatment (2.5wt% NaOH, 1.5wt% urea, and 8wt% sample biomass) was mixed for 40 minutes at 121°C, followed by enzymatic hydrolysis (2ml Novozymes Cellic glucadase enzyme and 5gram pretreated biomass) shaken at 50rpm for 40 minutes at 50°C, and separated by centrifuge at 8500rpm to hydrolyze Napier grass. Carbohydrates make up 52.1 % of the Napier grass composition, making it an excellent carbon source. Acetone-Butanol-Ethanol fermentation (ABE) was carried out in a continuous fermentation process using co-cultured C. acetobutylicum bacteria. After optimizing the operating parameters pH, Temperature, substrate concentration, and fermentation time, the productivity of butanol was found to be 0.077 g lt -1 hr -1 with a 29.5 % efficiency. The yield of ABE was 0.0833 g lt -1 hr -1 when compared to the semi–Simultaneous Saccharification fermentation (sSSF) process, which combined alkaline pretreated biomass with enzymatic hydrolysis and cocultured bacteria after 24 hours of hydrolysis followed by 96hrs of fermentation. Butanol efficiency at the s-SSF process is 33.6 %. When compared to previous studies, ABE fermentation of Napier grass yields a competitive yield. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/32055 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Napier grass | en_US |
| dc.subject | Alkali-pretreatmentl | en_US |
| dc.subject | Enzymatic hydrolysis | en_US |
| dc.subject | Acetone-Butanol-Ethanol (ABE) fermentationl | en_US |
| dc.subject | semi-Simultaneous Saccharification fermentation (sSSF) | en_US |
| dc.subject | Bio-Butanol | en_US |
| dc.title | Bio-Butanol Production from Napier Grass Hydrolysis Using Clostridium Acetobutylicum | en_US |
| dc.type | Thesis | en_US |