Bovine Tuberculosis Lesion Description with Molecular Characterization of Mycobacterium Species from Cattle Slaughtered at Abattoirs Bishoftu, Ethiopia
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Date
2016-06
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Addis Ababauniversity
Abstract
A cross-sectional study was conducted at ELFORA and Bishoftu Municipality Abattoir from November 2015 to April 2016 to describe the gross and microscopic lesion of bovine tuberculosis and characterize its causative agents. Post-mortem examination, pathological scoring, bacteriological culturing, Zeihl Neelson staining, histopathology and multiplex polymerase chain reaction (PCR) were used for investigation. Six hundred twenty six (626) Cattle were recruited for the study and the prevalence was found to be 2.9% (18/626) on the basis of gross lesion. There was statistical significant (X2=24.01 and P=0.000) difference between age of the animal and tuberculosis lesion. Eighty eight percent (88%) of the gross lesion was detected in the thoracic cavity while 12% of the lesion was found in the abdominal cavity and head region. Only twenty eight percent (28%=5/18) of the suspicious lesions yielded colonies of which one were acid-fast positive. Severe depletion of lymphocytes, calcification, cellular debris and aggregation of macrophage were observed microscopically. Further identification of the isolates using multiplex PCR revealed that the isolates belong to the Genus Mycobacterium. Of the isolates that showed signal to the Genus Mycobacterium, it was the members of the non-tuberculosis Mycobacterium complex. In conclusion, TB lesions were caused by the non-tuberculosis Mycobacterium complex. Hence, as the isolates were the non-tuberculosis Mycobacterium complex members, the pathogenecity and public health importance of these members in cattle needs further study.
Key words: Bishoftu, Bovine tuberculosis, Calcifications, Cattle, Non-tuberculosis Mycobacterium, PCR
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Keywords
Bishoftu, Bovine tuberculosis, Calcifications, Cattle, Non-tuberculosis Mycobacterium PCR