Antibiotic Resistance Patterns of Gram Negative Fermentative and non Bacilli isolated from patients Referred to Arsho Advanced Medical Laboratory Addis Ababa, Ethiopia

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Addis Ababa University


Background: Gram negative bacilli are the most frequently isolated bacteria which recovered from various clinical samples. Conventionally, these microorganisms subdivided into two major groups’ fermentative and non-fermentative gram-negative bacteria. The aim of this study is to determine antibiotic resistance patterns of fermentative and non-fermentative gram negative bacilli from various clinical samples in study area. Method: A laboratory based cross-sectional study conducted at Arsho Advanced Medical laboratory, Addis Ababa, Ethiopia from January to April 2017; Study participants were required Urine , CSF, wound, Nasal fluid ,Ear ,Blood, Body fluid, Semen were collected and cultured on Blood agar, MacConkey agar and chocolate agar and incubated according to standard conditions. Then Species identification and antimicrobial susceptibility testing of gram negative bacteria were determined by automated Vitek 2 compact system using GN and AST GN72 card. Result: From the total of 824 various specimens, 284(34.5%) were culture positive , of which (90.8%, 258/284) of the isolates were fermentative and (9.2%), 26/284) were non fermentative Gram- negative bacteria from wide range of clinical specimens such as in urine 197(69.4%), in wound and abscess 34(11.9%), in Ear 20(7.0%), in CSF 11(3.87%), in Nasal 11(3.9%), in Blood 4 (1.4%), in Bodyfluid4 (1.4%) and in semen 3(1.1%) .The most common Gram- negative bacterial isolates being Escherichia coli 152(53.5%), followed by Klebsiella pneumonia 40(14.1%), Pseudomonas aeruginosa 13(4.6%), Proteus mirabilis 13(4.6%), Acinetobacter baumannii 8(2.8%) were the most dominant isolate respectively. Among gram negative bacteria, K.pneumoniae (82.5%), E.coli (82.9%), P.aeruginosa (100%), and A. baumanii (100%) were multi drug resistance. Concussion: High antimicrobial resistance and multi drug resistance was demonstrated over the study period, which may be due to associated with the improper antibiotic consumption or lack of a proper guideline for empirical therapy. Keywords: Antibiogram, Enterobacteriaceae, fermentative and non-fermenting gram-negative bacilli, antimicrobial susceptibility test and antimicrobial resistant



Antibiogram, Enterobacteriaceae, Fermentative