Antibiotic Resistance Patterns of Gram Negative Fermentative and non Bacilli isolated from patients Referred to Arsho Advanced Medical Laboratory Addis Ababa, Ethiopia
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Date
2017-06
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Addis Ababa University
Abstract
Background: Gram negative bacilli are the most frequently isolated bacteria which recoveredfrom various clinical samples. Conventionally, these microorganisms subdivided into two majorgroups’ fermentative and non-fermentative gram-negative bacteria. The aim of this study is todetermine antibiotic resistance patterns of fermentative and non-fermentative gram negativebacilli from various clinical samples in study area.
Method: A laboratory based cross-sectional study conducted at Arsho Advanced Medicallaboratory,AddisAbaba,EthiopiafromJanuarytoApril2017;Study participants were required Urine , CSF, wound, Nasal fluid ,Ear ,Blood, Body fluid,Semen were collected and cultured on Blood agar, MacConkey agar and chocolate agar andincubated according to standard conditions. Then Species identification and antimicrobialsusceptibility testing of gram negative bacteria were determined by automated Vitek 2 compactsystem using GN and AST GN72 card.
Result: From the total of 824 various specimens, 284(34.5%) were culture positive , of which (90.8%,258/284) of the isolates were fermentative and (9.2%), 26/284) were non fermentative Gram-negative bacteria from wide range of clinical specimens such as in urine 197(69.4%), in wound andabscess 34(11.9%), in Ear 20(7.0%), in CSF 11(3.87%), in Nasal 11(3.9%), in Blood 4 (1.4%),in Bodyfluid4 (1.4%) and in semen 3(1.1%) .The most common Gram- negative bacterial isolatesbeing Escherichia coli 152(53.5%), followed by Klebsiella pneumonia 40(14.1%), Pseudomonasaeruginosa 13(4.6%), Proteus mirabilis 13(4.6%), Acinetobacter baumannii 8(2.8%) were themost dominant isolate respectively. Among gram negative bacteria, K.pneumoniae (82.5%),E.coli (82.9%), P.aeruginosa (100%), and A. baumanii (100%) were multi drug resistance.
Concussion: High antimicrobial resistance and multi drug resistance was demonstrated over thestudy period, which may be due to associated with the improper antibiotic consumption or lackof a proper guideline for empirical therapy.
Keywords: Antibiogram, Enterobacteriaceae, fermentative and non-fermenting gram-negativebacilli, antimicrobial susceptibility test and antimicrobial resistant
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Antibiogram Enterobacteriacea Fermentative