Development of Edible Packaging Film from Nigella Sativa Seed Extract and Evaluation of Its Antimicrobial Activities Against Food – Born Pathogens
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Date
2019-12
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Addis Ababa University
Abstract
Edible packaging films have received a considerable attention in recent years because of their
advantage over the synthetic films in the reduction of environmental pollution. The aim of the
research was development of optimal edible packaging bio-films from gelatin and glycerol by
varying the concentration of (5,10 and 15 wt/wt) and (4,8,12 and 16v/wt) respectively with
the main responses of physical property (solubility, swelling and WVTR) and mechanical
property (tensile strength and elongation at break), and re-preparing a packaging bio-film by
adding Nigella Sativa seed extract together with gelatin and glycerol optimal response results
and finding its antimicrobial activities of the developed packaging bio-films against foodborn
pathogens. The factorial response optimizer results were gelatin concentration (10g
gelatin/100ml aqueous solution) and glycerol concentration (4ml glycerol/100ml aqueous
solution), solubility (54.76%), swelling (43.99%), WVTR (0.145gm/day cm
), tensile
strength (20.302Mpa), and elongation at break (14.95%). The optimal re-prepared packaging
bio-films with the addition of Nigella Sativa seed extract with the concentration (12.5%, 25%
and 50%) were also analyzed its Antimicrobial activities against targeted food-born
pathogens (Escherchia coli, Salmonella typhi, Staphylococcus aureus, and Pseudomonas
aeruginosa). The Nigella Sativa seed extract showed result (30, 23 and 19.5mm inhibition
zone on Escherchia coli , 30, 25.5 and 20mm inhibition zone on Salmonella typhi and 30, 24
and 20 inhibition zone on Staphylococcus aureus with the concentration of Nigella Sativa
seeds extract of 75, 50 and 25% for the mentioned pathogens respectively) except for
(Pseudomonas aeruginosa) whereas the bio-film that contain the Nigella Sativa seed extract
showed only surface inhibition. In conclusion, this study confirms that the bio-films
developed from this gelatin, glycerol and Nigella Sativa seed extract had an antimicrobial
activity on food-born pathogens. Therefore, this may be used for wide range of food
packaging on perishable vegetables and fruits in order to reduce their vulnerable microbial
growth and extend their shelf stability of food products.
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Keywords
Gelatin, Glycerol, Physical property, Mechanical property, WVTR, Antimicrobial activity, Nigella Sativa seed extract, Edible packaging bio-film, Food-born pathogens