Antihyperglycemic and antihyperlipidemic effect of Persea americana mill fruit juice in high fat diet and low dose streptozotocin (STZ) induced type 2 diabetic male albino Wistar rats
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Date
2020-06
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Addis Abeba University
Abstract
Introduction:Type II diabetes is an alarming rate problem globally and in Ethiopia due to
change in diatery habits and sedentary life style. Serious complications are associated with
diabetes, particularly macrovascular and microvascular complications. Early controlling blood
glucose significantly reduces the risk of complications of diabetes, but yet no effective cure for
it, even though available drugs currently used in managing the disease are associated with
several undesirable side effects. Traditional treatment with low cost and minimum side effect is
used to treat diabetes across the world due to the different types of ingredients present in
medicinal plants that act on a variety of targets by various modes and mechanisms. Ethiopia is
rich in traditional medicinal plants and Persea americana mill is also part of this which people
comsume as food.
Objectives: In this study the effect of Persea americana mill fruit juice on FBG, weight, LDL-c,
TG, TC, HDL-c and total protein in HFD/ low dose STZ indued type II diabetes mellitus has
been evaluated in male albino wistar rats.
Materials and Methods: Thirty six male albino wistar rats of 150-200 g weight were divided in
to six different groups. Except six rats, the rest thirty fed on HFD for one month to induce
prediabetes and insulin resistance, next followed by 35mg/Kg of STZ injection. Group I served
as normal control; Group II served as diabetic control; Group III recieved 7mg/Kg of metformin;
Group IV, V and VI were given 856 mg/kg, 1712 mg/kg and 2568 mg/kg of Persea americana
mill fruit pulp juice for six weeks respectivily. The effect of fruit pulp juice on food intake and
weight was measured by triple beam balance. After forty-five days treatment, the rats were
fasted overnight (12 to 14 hours), anaesthetized and blood sample was collected by cardiac
puncture for biochemical tests. Results were analyzed using one way ANOVA at a 5% level of
significance.
Results: In higher dose (2568 mg/Kg) of Persea americana mill fruit pulp treated group food
consumption,weight, FBG, LDL-c were signifacantly reduced and HDL-c increased (P<0.005)
compared to diabetic control. Middle dose (1712mg/Kg) treated group showed a decrease in
FBG on 6
th
week and improve HDL-c. Treatement of rats with Persea americana fruit juice
change TG ,total protien and creatinine but not significant. Oral antidiabetes drug metformin
showed significant reduction on pellet consumption, weight, FBG and lipid profile.
Conclusion:These results indicated that Persea americana mill fruit juice has antihyperglycemic
and antilipidemic effect possibly through reduction of fasting blood glucose, LDL-C and
increasing HDL-C, in T2D induced rats. .
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Keywords
T2DM, Persea americana mill, HFD, STZ, hyperglycemea, dyslipidemea