Pathological, Bacteriological and Serological Study on Pneumonic Sheep and Goats With Special Emphasis on Mannheimia, Babersteinia, Pasteurella and Mycoplasma Species
| dc.contributor.advisor | Bulto Giro | |
| dc.contributor.author | Tilaye Demissie | |
| dc.date.accessioned | 2026-08-04T10:07:57Z | |
| dc.date.available | 2026-08-04T10:07:57Z | |
| dc.date.issued | 2012-04-30 | |
| dc.description.abstract | A prospective, non-probability and purposive type study was conducted from October to April on pneumonic sheep and goats to iso late and characterize Mannheimia, Babersteinia, Pasteurella and Mycoplasma species involved in pneumonia of sheep and goats. The concurrent occurrence of Mannheimia, Babersteinia, Pasteurella and Mycoplasma species together were studied and Mannheimia haemolytica isolates were strain serotyped. Comparative isolation of Mannheimia, Babersteinia and Pasteurella species from the same samples of lung was performed. Conventional bacteriological isolation and identification procedures (Quinn et al., 2002; Sisay and Zerihun, 2003 and OlE, 2004) were used for isolation and identification of Mannheimia, Babersteinia and Pasteurella species. Isolation and identification of Mycoplasma species was conducted according to Miles and Nicholas (1998) and OlE (2008).Growth inhibition by monospecific hyperimmune sera and PCR techniques were used for Mycoplasma species identification. The total isolation rate fi'om pneumonic lung from both sheep and goats were 28 % (78/279) for Mannheimia haemolytica, 3.9 % (11 /279) for Babersteinia trehalose and 2.2% (6/279) for Pasteurella /IIultocida. Total isolation rate of Mannheimia, Babersteinia and Pasteurella species together was 40.7% from pneumonic sheep and 24. I % from pneumonic goats. Serotyping 0 f Mannheimia haemolytica isolates was carried out using IHA against 10 reference antisera. Serotype A2 (2 \.5%), AS (20 .5%), and A I (17.7%) were the three dominant serotypes from total isolates of both species. Serotype A2 (26.3%), A1 (17.5%), A8 (15.S/5%) were dominant serotypes from sheep isolates and serotypes A8(33 .3%), A1(19%), and A1(14%) were the dominat serotypes isolated from goat. Serotype AI3 was not isolated both from sheep and from goats and serotypes A8, A7, and A12 were not isolated from goats in the present study. goats in the present study. Four Mycoplasma species namely, Mycoplasma ovipnemoniae (9 isolates), MmmLC (2 isolates), Mmc (I iso late) and Mycoplasma arginini (2 isolates) were identified from sheep. Five Mycoplasma species namely, MmmLC (9 isolates), Mcc (3 isolates), Mccp (3isoaltes), Mmc (4 isolates) and M. arginini (2 isolates) were isolated and identified from goats. The concurrent iso lation rate of Mannheimia haemolytica and Mycoplasma species was 24.6% in sheep and 9.5% in goats. Mycoplasma ovipneumoniae was isolated at IS.S% rate with Mannheimia haemolytica in sheep. This was the highest concurrent occurrence observed while compared to other Mycoplasrna species isolated from Mannheimia haemolytica positive lungs. MmmLC, Mmc and Mycoplasma arginini were also isolated from Mannheimia haemolytica positive sheep. Generally, the isolation rate of Mycoplasma and Mannheimia haemolytica together was significantly lower in goats than in sheep and the only Mycoplasma species isolated together with Mannheimia haemolytica from goats was Mmc. Finally, the results are discussed and compared with previous work. Some points on control strategies and areas of further research are also suggested . | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/8740 | |
| dc.language.iso | en | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Antisera/BabersleinialDebreZeit/Ethiopia/Mannheimial Mycoplasmal Pasteurella/Pathological/Pneumonic lung IRatel Serotype. | |
| dc.title | Pathological, Bacteriological and Serological Study on Pneumonic Sheep and Goats With Special Emphasis on Mannheimia, Babersteinia, Pasteurella and Mycoplasma Species | |
| dc.type | Thesis |