Multidrug Resistance and Carbapenemase Producing Enterobacteriaceae on Clinical Samples Referred to Wudassie Advanced Medical Laboratory, Addis Ababa, Ethiopia
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Date
2025-07
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Addis Ababa University
Abstract
Background: One of the largest risks to world health is the spread of microorganisms that areresistant to drugs. According to estimates, themortality rate from Carbapenem-resistant Klebsiellapneumonia (CRKP) infections ranges from 37.2% to 42.1%. In 2019, Ethiopia reported 21,200deaths directly attributable to Anti-microbial resistance.
Objectives: To assess multidrug resistance and carbapenemase producing Enterobacteriaceae onclinical samples referred to Wudassie Advanced Medical Laboratory.
Method: A cross-sectional study was conducted from December 2024 to March 2025. A total of1105 patients from which 439 were suspected of sepsis, 110 suspected of wound infection, 45suspected of meningitis, 353 suspected of urinary tract infection and 153 suspected of otherinfections, whose specimens were sent to wudassie advanced medical laboratory during the studyperiod were included in this study. Isolates were identified using Matrix-Assisted Laser Desorption& Ionization- Time of Flight (MALDI-TOF) (EXS 3000, Zybio Inc., China). Antimicrobialsusceptibility testing was performed using the Kirby-Bauer disk diffusion technique and carbapenemase production was confirmed by modified carbapenem inactivation technique. Data entered using epi data version 4.6 and analyzed using Statistical Package for social sciencesstatistical software version 26 and the result was displayed with graphs, tables and words andcompared with previous studies.
Result: Out of 1105 eligible patients, 288 of them had microbial growth. Of this,156 of them wereEnterobacteriaceae. The predominant isolate was E. coli followed by K. pneumoniae and P.mirabilis. From 156 Enterobacteriaceae isolated, 78.8% of them were multidrug resistant and19.5% of them were carbapenemase producing Enterobacteriaceae (CPE). K. pneumoniae wasthe most abundant CPE isolate. Most of CPE producing isolates were from urine specimenfollowed by wound and sputum specimen.
Conclusion: The findings highlights a high prevalence of multidrug-resistance and a rise in theprevalence of carbapenemase-producing Enterobacteriaceae, Thus, it is important to reviewcurrent treatment guidelines for better patient management and outcome.
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Keywords
Enterobacteriaceae Multidrug resistance Carbapenemase production