Magnitude of Pediatric Sepsis and Antimicrobial Resistance at Adama Medical College Hospital, Adama, Ethiopia.
| dc.contributor.advisor | Hailu, Melese (PhD) | |
| dc.contributor.advisor | Diriba, Regasa | |
| dc.contributor.author | Redwan, Megfira | |
| dc.date.accessioned | 2026-01-30T11:44:36Z | |
| dc.date.available | 2026-01-30T11:44:36Z | |
| dc.date.issued | 2025-05 | |
| dc.description.abstract | Background: Pediatric sepsis and antimicrobial resistance are a leading cause of severe illness and death among children, especially in developing countries. Objective: The aim of this study was to determine the magnitude of pediatric sepsis and antimicrobial resistance at Adama Medical College Hospital, Ethiopia. Methods: A cross-sectional study was conducted at Adama Hospital Medical College from March 2024 to January 2025. Blood was collected for culture in aseptic techniques and Tryptic Soya Broth was used for inoculation. Following incubation, subculturing was performed on blood agar, chocolate agar and MacConkey agar plates. For samples that showed bacterial growth, identification was carried out based on colony morphology, Gram staining, and standard biochemical tests. Once the bacterial species were identified, antibiotic susceptibility testing was performed using the disk diffusion methods. Additional tests were conducted to detect extended-spectrum beta-lactamase (ESBL) production, carbapenemase resistance, and methicillin-resistant Staphylococcus aureus (MRSA). Statistical analysis was performed using SPSS version 26. Result: Among 400 blood cultures done, 144 (36%) showed bacterial growth. The bacteria that were most dominant in this study were Staphylococcus aureus 36 (25%), Acinetobacter baumannii 25 (17.4%), Enterobacter cloacae 13 (9%), Klebsiella pneumoniae 8 (5.6%), and Serratia marcescens 8 (5.6%). Enterobacteriaceae most often showed resistance to ampicillin (94.1%), cefotaxime (89.2%), cefepime (86.2%), and ceftazidime (84.6%). MDR frequency of Enterobacteriaceae was 87%, and Gram-positive was 33.3%. MRSA was found to be 88.9%, ESBL production was detected in 87.5% of Citrobacter koseri (n=8), 85.7% in Providencia stuartii (n=7), and 83.3% in Enterobacter cloacae (n=12), and carbapenemase resistance was detected in 100% of Pantoea agglomerans (n=2) and 50% of Acinetobacter baumannii (n=6). Conclusion: The prevalence of pediatric sepsis and the level of multidrug resistance (MDR) among the children studied were notably high. However, certain antibiotics from the carbapenem class, along with amikacin, demonstrated effective antimicrobial activity. The rates of methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL)-producing organisms were also significantly higher than expected. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/7612 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Pediatric | |
| dc.subject | sepsis | |
| dc.subject | ESBL | |
| dc.subject | MRSA | |
| dc.subject | carbapenemase | |
| dc.subject | AMR | |
| dc.subject | Ethiopia | |
| dc.title | Magnitude of Pediatric Sepsis and Antimicrobial Resistance at Adama Medical College Hospital, Adama, Ethiopia. | |
| dc.type | Thesis |