Detection and determination of tetracycline residue levels in beefg at Addis Ababa, D/Z, & Nazareth Slaughtered houses
No Thumbnail Available
Date
2007-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A cross-sectional study was conducted from October 2006 to May 2007 to investigate the
proportion of tetracycline residue levels in beef at Addis Ababa, Debre Zeit and Nazareth
slaughterhouses. A total of 384 muscle and kidney samples were randomly collected from
slaughtered cattle in the respective slaughterhouses. The samples were qualitatively screened for
tetracycline residues by thin layer chromatography using a suitable silica gel (Merck silica gel 60
plates).The positive samples were then further analyzed by using high performance liquid
chromatography (HPLC) Shimadzu Model Class-VP series with an electron diode array detector.
The result of this study indicated that oxytetracycline residues previously detected in thin layer
chromatography were detected in all samples by HPLC. A given sample was regarded as positive
for tetracycline if its retention time and peak corresponded to that of the standard. Out of the 384
samples that were analyzed for tetracycline residues 274 (71.35%) had detectable oxytetracycline
residues. Tetracycline and doxycycline were not detected. At Addis Ababa slaughterhouse, 120
(93.75%) of kidney and beef samples were positive for oxytetracycline. In Debre Zeit 48 (37.5%)
of kidney and beef samples were also positive for oxytetracycline while from Nazareth
slaughterhouse 106 (82.81%) of kidney and beef were positive for oxytetracycline. The mean
(p>0.05) residue levels of oxytetracycline for the three slaughterhouses studied in muscle were as
follows: Addis Ababa 108.34μg/kg, Nazareth 64.85μg/kg and 15.916μg/kg at Debre Zeit while in
kidney samples were (p<0.05): 99.02 μg/kg in Addis Ababa, 109.35μg/kg in Nazareth and
112.53μg/kg in Debre Zeit slaughterhouses. The oxytetracycline positive samples, which showed
residues of oxytetracycline above maximum residue limits (100μg/kg) in muscle samples, were
58 (48.33%) at Addis Ababa slaughterhouse and 51 (48.11%) at Nazareth slaughterhouse and 1
(0.9%) in the kidney samples of Nazareth slaughterhouse. At Debre Zeit slaughterhouse no
samples were above the maximum residue limit. The results obtained confirmed that
oxytetracycline was heavily and indiscriminately used in the fattening farms, small scale
fattening farms, pastoralists and other animal owners. The results also suggest that the
recommended withdrawal time was not strictly applied. Results of the present study could serve
as a preliminary base line information for veterinary authorities, drug administration and quality
control authority, other concerned organizations and professionals to take measures on control
x
and prevention of drug residues; as well as the consumer to be aware of the possible drug residue
risks through consumption of unsafe animal products.
Description
Keywords
Tetracycline, residue, beef, kidney