Determination of Hematological Reference Interval for Apparently Healthy Children in Addis Ababa, Ethiopia

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Addis Abeba University


Background: Clinical laboratory reference intervals are an important tool to identify abnormal laboratory test results. The generating of hematological parameters reference intervals for local population is very crucial to improve quality of health care, which otherwise may lead to unnecessary expenditure or denying care for the needy. There are no well-established reference intervals for hematological parameters in Addis Ababa, Ethiopia. Objective: To determine for apparently healthy children hematological reference Interval for apparently healthy children in Addis Ababa, Ethiopia. Methods: A community based cross-sectional study design was employed involving 272 individual in Addis Ababa Ethiopia from April–October 2019. Blood samples of 10 ml was collected in EDTA tubes using multi sample needle. A complete blood count (CBC) was performed using Sysmex automated hematology analyzer. The data was analyzed by SPSS version 26 statistical software and a non-parametric test used to generate reference intervals based on the 2.5th and 97.5th percentiles of the data based on CLSI guideline. Result: The reference interval of RBC count, WBC count, HB and PLT count were 4.235.59x10 12 /L, 2.95-10.89x10 9 /L, 12.02-16.04/dl and 208.90-538.9x10 9 /L, respectively for the age category ( 5-11) while 4.43-5.85x10 12 /L, 3.05-9.43x10 9 /L,13.2-16.94g/dl, ,202.01-459.92x10 /L for age group (12-14) respectively. Most of hematological parameters showed significant differences such as RBC, HGB, HCT and MCV. No significant age-specific differences were observed for MCH or MCHC, WBC and Platelet parameters. Conclusion: This study provided population-specific hematological reference interval for children in Addis Ababa, Ethiopian.



Reference interval, hematological parameter, children, Addis Ababa Ethiopia