Toxicity study of anti-ectoparasiticformulation comprising Eucalyptus globulus and Jatrophacurcas oils blended using industry based emulsifier on the histopathology of liver, kidneys and some blood parameters in mice.

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Addis Ababa Universty


Background: Agriculture delivers a livelihood for the people of many African countries. Accordingly, animal agriculture provides strong base for Ethiopian economy. However, the presence of ectoparasites and secondary infections caused by their infestations decrease the quantity and quality of livestock production leading to low income. A lot of chemicals such as organophosphates, carbamates, acaricides, and chlorinated hydrocarbons are widely applied to regulate these ectoparasites and their adverse effects. However, most synthetic anti-ectoparasites are toxic and the relatively safe chemicals are expensive. Utilization of affordable and safer products from natural sources is, therefore, highly commendable. Objectives: The present study was carried out to evaluate the acute and sub-chronic toxic effects of the formulation. Methods: The experiments were performed on a total of 72 healthy male and female (54 for acute and 18 for sub-chronic) Swiss Albino mice. Grouping was done randomly based on the OECD guideline. The treatment groups were orally administered with 1.25%, 1.9%, 2.9%, 4.4%, 6.6%, 9.9%, 14.9% and 20% ml/kg body weight doses of the formulation for the acute toxicity studies. 1.25% and 3.75% ml/kg body weight doses of the formulation were used for the subchronic toxicity studies. The control groups were administered with distilled water during both acute and sub-chronic toxicity studies. Results: There was no change in the general behavior of treatment groups as compared to the control during acute toxicity study. No death was recorded. The LD50 was found higher than 20% ml/kg body weight dose of the formulation. In the sub-chronic studies, no significant biochemical, hematological and body weight changes were observed, except for LDL, which was found increased in both treatment groups. Generally, histological architecture of liver and kidneys were normal. However, liver of animals treated with dose of 3.75% ml/kg showed small number of mononuclear leukocytic infiltrations around portal areas. Minor tubulointerstitial leukocytic infiltrations were also observed in the kidney sections of these animals. Conclusion and Recommendations: Results of the acute and sub-chronic toxicity studies revealed that the formulation is relatively safe. Further studies in other organs and animals are recommended toward establishing the safety of the formulation.



Eucalyptus globulus, Jatropha curcas, Ectoparasites, Toxicity, Swiss albino mice