Semen Quality, Fertility and Hatchability of in Vitro Liquid and Cryopreserved Semen Using Commercial and Homemade Extenders from Indigenous Horro Chicken Breed
| dc.contributor.advisor | Prof. Gebeyehu Goshu | |
| dc.contributor.advisor | Prof. Alemayehu Lemma | |
| dc.contributor.author | Tarekegn Getachew | |
| dc.date.accessioned | 2023-12-11T08:32:10Z | |
| dc.date.available | 2023-12-11T08:32:10Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | Short-term storage and cryopreservation of poultry semen represents an important strategy for breeding and in vitro conservation of the genetic material of chicken populations. The objective of this study is to evaluate the quality, fertility and hatchability of liquid stored and cryopreserved semen from Horro chicken using homemade tris-based extender and commercial extender. Furthermore, the economic benefits of artificial insemination were also evaluated for commercial broiler breeder farming. A total of 30 roosters and 160 adult hens were used for semen collection, and artificial insemination, respectively. Pooled semen samples were divided into three groups: Semen without extender; Semen diluted with homemade extender and Semen diluted with commercial extender. Semen was either liquid stored or cryopreserved in liquid nitrogen for shorter and longer-term storage, respectively. Changes in spermatozoa motility, in vitro viability, and morphology were evaluated in cryopreserved semen, and fresh diluted semen (1:4 v/v semen to extender) after 4hr, 8hr, 12hr, and 24hr storage at 4°C. Meta analysis on the effects of Glycerol and DMF as cryoprotectants in cryopreservation of chicken semen was conducted. Data was collected from electronic databases. Relevant data was extracted from studies such as sample size, breed, cryoprotectant used, post-thaw sperm quality and fertility. Data was presented using forest plots. Fresh semen collected from Horro chicken breed in this study was moderately viscous, milky, pH of 7.2 with 5.5x109 spermatozoa per ml of semen. The average ejaculate volume was 0.36 ml. There was a significant influence of temporary storage and cryopreservation on mass motility, morphological abnormality (with high incidence of the bent tail), and viability. Semen stored using BPSE for 4 hours significantly (P<0.05) found to be suitable for short-term storage of semen collected from Horro chicken breed with progressive sperm motility (87.0 ± 1.22) and %live sperm (83.6 ± 1.63a). There was significant decline observed in all sperm quality attributes in increasing storage duration. There were significant differences (P<0.05) in progressive sperm motility, mass motility, and in vitro viability between commercial and homemade extenders in cryopreserved semen. However, no significant difference was observed in mass motility across the extenders in cryopreserved semen collected from Horro Chicken breed. The commercial ASD extender was significantly (P<0.05) found to be the most suitable regarding the proportion of morphologically normal sperm (64.25 ± 0.91) and in vitro viability rate of cryopreserved sperm samples. There were no significant differences across all treatments in terms of fertility and hatchability rate. Results from meta-analysis show that, the highest result has been recorded in diluent prepared by Mehdipour et al., 2020a using 3.8% DMF in lake extender in Ross chicken breed (71.1 ± 2.01). The results showed locally prepared tris-egg yolk-based extender could be a suitable extender for short-term storage and cryopreservation of chicken sperm regarding the sperm quality attributes and fertility. Further studies are suggested to improve fertility and hatchability. | |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/597 | |
| dc.language.iso | en | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Cryopreservation | |
| dc.subject | Horro | |
| dc.subject | in vitro viability | |
| dc.subject | Motility | |
| dc.subject | Morphology | |
| dc.subject | Semen | |
| dc.title | Semen Quality, Fertility and Hatchability of in Vitro Liquid and Cryopreserved Semen Using Commercial and Homemade Extenders from Indigenous Horro Chicken Breed | |
| dc.type | Thesis |