Magnitude of Helicobacter Pylori Infection among Peptic Ulcer Disease patient of two private health facilities of Addis Abeba, Ethiopia: Evidence of stool Antigen test and staining of Biopsy Materials.

No Thumbnail Available



Journal Title

Journal ISSN

Volume Title


Addis Ababa University


BACKGROUND: Helicobacter pylori infection is one of the most common chronic bacterial infections of humans and has a worldwide distribution. Epidemiological studies strongly suggested that more than 50% of the world’s populations are colonized by H. pylori. Peptic ulcer disease (PUD) is the most common illness in the Ethiopian population visiting outpatient department of health facilities, and it has also been associated with H.pylori infection. The aim of this study was to assess the magnitude of H.pylori and its associated factors among peptic ulcer disease patients who visited at landmark general hospital and adera internal medical speciality center outpatient department. Around 70–90% of peptic ulcer disease (PUD) is due to helicobacter pylori and requires treatment with antimicrobials to which these bacteria are susceptible. OBJECTIVE: To assess the magnitude of helicobacter pylori infection among peptic ulcer diseases pateints of two private health facilites Addis Ababa, Ethiopia: evedence of stool antigen test and staining of biopsy materials METHODS: An institutional-based cross-sectional study was conducted on 150 peptic ulcer patients. Non-probability convenient sampling techniques were used to select study participants. Data were collected by using structured questionnaire via face-to-face interview. H.pylori infection was diagnosed using stool antigen test method. The data were entered into Epi info version 3.5.3 and transferred to Statistical Package for Social Sciences version 20. Both Bivariable and multivariable binary logistic regression analyses were performed to see the effect of independent variables on the dependent variable. RESULTS: Of the total study participants, 45(41%), 52(58%) and 36(43%) were married, rural residents and male, respectively. All peptic ulcer disease patients had abnormal histopathology findings. The overall magnitude of H.pylori infection was 52 %. In bivariable logistic regression analysis, sex and marital status were significantly associated with H.pylori infection. The sensitivity and specificity of H.pylori stool antigen test is 70.5 % while the sensitivity of giemsa and gram stain was 88.5 %. However, all methods have 100 % specificity. CONCLUSION: The magnitude of H.pylori infection is 52%. This is high indicating that it is a public health problem in the study area. According to this study, sex and marital status were vii | P a g e significantly associated with H.pylori infection. Hence, effective preventive, control and screening strategies need to be designed to reduce the burden of the disease.


This is to certify that the thesis prepared by Tewodros Kassahun entitled “MAGNITUDE OF HELICOBACTER PYLORI INFECTION AMONG PEPTIC ULCER DISEASES PATIENT OF TWO PRIVATE HEALTH FACILITES OF ADDIS ABABA, ETHIOPIA: EVIDENCE OF STOOL ANTIGEN TEST AND STAINING OF BIOPSY MATERIALS” and submmitted in partial fulfillment of the requirements for Master of Science degree in Clinical Laboratory Science (Diagnostic and Public Health Microbiology) complies with the regulations of the University and meets the accepted standards with respect to originality and quality.


PUD, Endoscopy, Helicobacter pylori stool antigen, sensitivity, specificity, Staining method