OCCURRENCE AND ANTIMICROBIAL SUSCEPTIBILIY PROFILE AMONG E. COLI O157: H7 ISOLATED FROM THE ABATTIOR, REATAIL SHOP AND DIARRHEIC PATIENT IN BISHOFTU TOWN, EAST SHOA ETHIOPIA
| dc.contributor.advisor | Dr.Fanta Desissa | |
| dc.contributor.author | ALEMNESH, JUFARE | |
| dc.date.accessioned | 2018-09-26T07:40:03Z | |
| dc.date.accessioned | 2023-11-08T11:38:07Z | |
| dc.date.available | 2018-09-26T07:40:03Z | |
| dc.date.available | 2023-11-08T11:38:07Z | |
| dc.date.issued | 2018-06 | |
| dc.description | A Thesis Submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Veterinary Science in Veterinary Public Health. By Alemnesh Jufare | en_US |
| dc.description.abstract | A cross-sectional study was conducted from December 2017 to May 2018 to investigate the occurrence and antimicrobial susceptibility profile of E. coli O157:H7 in cattle fecal samples at Bishoftu abattoir, meat at retail shops and stool of diarrheic patients at Bishoftu hospital. A total of 583 samples consisting 240 feces, 127 meats and 216 stool samples were collected using systematic sampling technique and analyzed according to the recommended standard procedure to isolate and identify the pathogen. In addition, questionnaire survey was conducted to assess the current status of hygienic and handling practices in the abattoir, retail shops and exposure assessments of diarrheic patients. Out of the total 583 samples examined, 31(5.3%) were positive for E. coli O157:H7. The prevalence of E. coli O157:H7 was 7% (95%CI: 3.81, 10.32), 6.3% (95%CI: 20.16, 10.58) and 2.8 (95%CI: 0.56, 4.99) in feces, meat and stools, respectively. However, statistically no significant difference was observed among the three sample types (X2 = 4.4969, P = 0.106). Based on univariate logistic regression analysis, all the considered variables at retail meat shops were not statistically associated with the occurrence of the pathogen (P>0.05). Among the risk factors considered the exposure of the diarrheic patients, only four days duration of onset of diarrhea was statistically associated with the occurrence of E. coli O157: H7 (OR: 9.51 (95% CI: 1.02, 88.00, P=0.047). The study also revealed that, a varying level of resistance of E. coli O157:H7 against the ten commonly used antimicrobials. All the isolates of E. coli O157:H7 were susceptible to gentamicine and resistant to ampicilin, cefoxitin and nitrofurantoin. Multi-drug resistance was observed in all the isolates. In conclusion, the study showed the occurrence of E. coli O157:H7 along beef supply chain with relatively high prevalence in cattle at abattoir and resistant E. coli O157:H7 to frequently used antimicrobials suggesting the need for intervention. Eventually, good hygienic practices along the beef supply chain and public education to safeguard the public from the associated risks and further study to identify the sources of the bacteria, establish the clear link between human diarrheal illness and beef consumption and investigating the genetic similarity of the isolates were recommended. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/12250 | |
| dc.language.iso | en | en_US |
| dc.subject | Antimicrobial susceptibility | en_US |
| dc.subject | Beef | en_US |
| dc.subject | E. coli O157:H7 | en_US |
| dc.title | OCCURRENCE AND ANTIMICROBIAL SUSCEPTIBILIY PROFILE AMONG E. COLI O157: H7 ISOLATED FROM THE ABATTIOR, REATAIL SHOP AND DIARRHEIC PATIENT IN BISHOFTU TOWN, EAST SHOA ETHIOPIA | en_US |
| dc.type | Thesis | en_US |